Abstract
In this study, a recently proposed NMR standardization approach by 2H integral of deuterated solvent for quantitative multicomponent analysis of complex mixtures is presented. As a proof of principle, the existing NMR routine for the analysis of Aloe vera products was modified. Instead of using absolute integrals of targeted compounds and internal standard (nicotinamide) from 1H-NMR spectra, quantification was performed based on the ratio of a particular 1H-NMR compound integral and 2H-NMR signal of deuterated solvent D2O. Validation characteristics (linearity, repeatability, accuracy) were evaluated and the results showed that the method has the same precision as internal standardization in case of multicomponent screening. Moreover, a dehydration process by freeze drying is not necessary for the new routine. Now, our NMR profiling of A. vera products needs only limited sample preparation and data processing. The new standardization methodology provides an appealing alternative for multicomponent NMR screening. In general, this novel approach, using standardization by 2H integral, benefits from reduced sample preparation steps and uncertainties, and is recommended in different application areas (purity determination, forensics, pharmaceutical analysis, etc.).
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References
B. Diehl, U. Holzgrabe, Y. Monakhova, T. Schönberger, J. Pharm. Biomed. Anal. 177, 112847 (2020)
G.A. Barding, R. Salditos, C.K. Larive, Anal. Bioanal. Chem. 404, 1165–1179 (2012)
S. Singh, R. Roy, Expert Opin. Drug Discov. 11, 695–706 (2016)
E. Hatzakis, Compr. Rev. Food Sci. Food Saf. 18, 189–220 (2019)
J. Ewanicki, W. Kim, W. Wang, Magn. Res. Chem. 58(8), 733–744 (2020)
Y. Monakhova, B. Diehl, Talanta 222, 121504 (2021)
Y.B. Monakhova, G. Randel, B.W.K. Diehl, J. AOAC Int. 99(5), 1213–1218 (2016)
U. Girreser, T. Ugolini, S.S. Çiçek, Talanta 205, 120109 (2019)
P. Jiao, Q. Jia, G. Randel, B. Diehl, S. Weaver, G. Milligan, J. AOAC Int. 99(5), 1213–1218 (2016)
Acknowledgements
Y. Monakhova acknowledges support of the Russian Science Foundation (project 18-73-10009).
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Monakhova, Y.B., Diehl, B.W.K. Simplification of NMR Workflows by Standardization Using 2H Integral of Deuterated Solvent as Applied to Aloe vera Preparations. Appl Magn Reson 52, 1591–1600 (2021). https://doi.org/10.1007/s00723-021-01393-4
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DOI: https://doi.org/10.1007/s00723-021-01393-4