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EPR Technology Spectroscopic Investigation of Generation of Reactive Oxygen Species Free Radicals in Laccase-Activated Jute Fiber

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Abstract

Lignins in jute fibers can be activated by laccase to generate reactive oxygen species (ROS) free radicals for subsequent use in radical graft polymerization. The effect of incubation conditions on the generation of ROS free radicals when jute powders were incubated with laccase was investigated through spin-trapping technique to detect the spin adduct of N-tert-butyl-α-phenylnitrone and ROS free radical using electron paramagnetic resonance spectroscopy. Results revealed that the highest concentrations of ROS free radicals were generated when jute was incubated with laccase (0.54 U/mL) and copper ions (10 mM) in solution at pH 3.5 and a temperature of 60 °C for 1 h.

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Acknowledgments

This work was financially supported by National Natural Science Foundation of China (51173071, 21274055), Program for New Century Excellent Talents in University (NCET-12-0883), the Natural Science Foundation of Jiangsu Province (BK2011157), the Fundamental Research Funds for the Central Universities (JUSRP51312B) and Program for Changjiang Scholars and Innovative Research Team in University (IRT1135). We thank Professor Zhao Baolu from the Institute of Biophysics, Chinese Academy of Sciences for his review and proposed amendments.

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Authors and Affiliations

  1. Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi, 214122, Jiangsu, China

    Chunxiao Zhou, Aixue Dong, Qiang Wang & Xuerong Fan

  2. Nantong Vocational College, Nantong, 226007, Jiangsu, China

    Chunxiao Zhou

  3. Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

    Yuanlin Cao

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  1. Chunxiao Zhou
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  2. Aixue Dong
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  3. Qiang Wang
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  4. Xuerong Fan
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  5. Yuanlin Cao
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Correspondence to Xuerong Fan or Yuanlin Cao.

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Zhou, C., Dong, A., Wang, Q. et al. EPR Technology Spectroscopic Investigation of Generation of Reactive Oxygen Species Free Radicals in Laccase-Activated Jute Fiber. Appl Magn Reson 45, 505–515 (2014). https://doi.org/10.1007/s00723-014-0534-6

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  • DOI: https://doi.org/10.1007/s00723-014-0534-6

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