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Molecular Motion in Frozen Phospholipid Bilayers in the Presence of Sucrose and Sorbitol Studied by the Spin-Echo EPR of Spin Labels

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Abstract

Electron spin echo (ESE) spectroscopy, a pulsed version of electron paramagnetic resonance (EPR), was applied to spin-labeled stearic acids in phospholipid bilayers hydrated in the presence of sucrose and sorbitol, which are known for their cryoprotective action on biological membranes. The phospholipids were 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). Stearic acids were labeled by nitroxide 4,4-dimethyl-oxazolidine-1-oxyl (DOXYL) attached rigidly at either the 5th or 16th specific carbon positions. ESE detects fast stochastic small-angle restricted molecular rotations (stochastic molecular librations) with correlation times on the nanosecond timescale. These motions are believed to have the same nature as the anharmonic motions of hydrogen atoms in biological substances detected by neutron scattering and Mössbauer spectroscopy, which become active above 200 K. To ensure that the echo decays indeed originate from fast stochastic molecular librations, a three-pulse stimulated spin echo was employed. It was found that the presence of sucrose or sorbitol suppresses the observed molecular motions. The observed effect was nearly the same for both label positions, indicating that the motions are similarly suppressed near the bilayer surface and in the bilayer interior. This finding suggests non-specific interactions of sugars with bilayer surface, which are likely to influence only the bulk physical properties of hydrated membranes. The results obtained show the usefulness of spin-echo EPR of spin labels when applied to investigate the molecular mechanisms of action of cryoprotective agents on biological systems.

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Acknowledgments

We are grateful to N.P. Isaev for his assistance with the experiments. Our work was supported by the Russian Foundation for Basic Research (grant no. 12-03-00192) and the Ministry of Education and Science of the Russian Federation (contract no. 11.519.11.1006).

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Authors and Affiliations

  1. Novosibirsk State University, Novosibirsk, 630090, Russia

    Nikita V. Ivanisenko & Sergei A. Dzuba

  2. Voevodsky Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    Nikita V. Ivanisenko & Sergei A. Dzuba

Authors
  1. Nikita V. Ivanisenko
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  2. Sergei A. Dzuba
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Correspondence to Sergei A. Dzuba.

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Ivanisenko, N.V., Dzuba, S.A. Molecular Motion in Frozen Phospholipid Bilayers in the Presence of Sucrose and Sorbitol Studied by the Spin-Echo EPR of Spin Labels. Appl Magn Reson 44, 883–891 (2013). https://doi.org/10.1007/s00723-012-0436-4

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  • DOI: https://doi.org/10.1007/s00723-012-0436-4

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