Abstract
In plant tissue culture, differences in endogenous levels of species-specific plant growth regulators (PGRs) may explain differences in regenerative capacity. In the case of polyamines (PAs), their dynamics and distribution may vary between species, genotypes, tissues, and developmental pathways, such as sexual reproduction and apomixis. In this study, for the first time, we aimed to assess the impact of varying endogenous PAs levels in seeds from distinct reproductive modes in Miconia spp. (Melastomataceae), on their in vitro regenerative capacity. We quantified the free PAs endogenous content in seeds of Miconia australis (obligate apomictic), Miconia hyemalis (facultative apomictic), and Miconia sellowiana (sexual) and evaluated their in vitro regenerative potential in WPM culture medium supplemented with a combination of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The morphogenic responses were characterized by light microscopy and scanning electron microscopy and discussed regarding the endogenous PAs profiles found. Seeds of M. sellowiana presented approximately eight times more putrescine than M. australis, which was associated with a higher percentage of regenerated calluses (76.67%) than M. australis (5.56%). On the other hand, spermine levels were significantly higher in M. australis. Spermine is indicated as an inhibitor of auxin-carrying gene expression, which may have contributed to its lower regenerative capacity under the tested conditions. These findings provide important insights into in vitro morphogenesis mechanisms in Miconia and highlight the significance of endogenous PA levels in plant regeneration. These discoveries can potentially optimize future regeneration protocols in Miconia, a plant group still underexplored in this area.
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10 April 2024
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The authors are also grateful to the “Centro de Microscopia Eletrônica (CME) da UFPR” for support in SEM analyzes.
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This research was supported by funds from “Pró-Reitoria de Pesquisa e Pós-Graduação da Universidade Federal do Paraná” (UFPR-PRPPG-04/2019) and “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior” (CAPES-Brazil; grant to JKZ (#88887.373597/2019-00).
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JKZ, LNV, RG, and HPFF designed the research; TRO and CS carried out the polyamine analyses; JKZ carried out the morphoanatomical analyses. All the authors read, reviewed, and approved the manuscript.
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Ziemmer, J.K., dos Reis de Oliveira, T., Santa-Catarina, C. et al. Plant regeneration capacity in seeds of three species of Miconia (Melastomataceae) may be related to endogenous polyamine profiles. Protoplasma (2024). https://doi.org/10.1007/s00709-024-01945-y
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DOI: https://doi.org/10.1007/s00709-024-01945-y