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Myo-inositol phosphate synthase improves heat stress tolerance by ethylene-mediated modulation of chlorophyll content and photosynthetic efficiency

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Abstract

L-myo-inositol phosphate synthase (MIPS; EC 5.5.1.4) encodes the enzyme synthesizing Myo-inositol for plant growth and development. Myo-inositol and its phosphate derivatives are involved in various physiological functions ranging from cell wall synthesis, chromatin remodeling, signal transduction, and providing stress responses. In the present study, we report that MIPS regulates chlorophyll content and photosynthesis efficiency via the ethylene signaling pathway. We have used Triticum aestivum MIPS-A (TAMIPS-A) for the present study and characterized it by mutant complementation and overexpression studies in Arabidopsis. TaMIPS-A overexpressing Arabidopsis transgenics were analyzed physiologically under thermal stress conditions. Analysis of overexpression TaMIPS-A transgenics under control and thermal stress conditions revealed them to have enhanced photosynthetic potential under heat stress. When TaMIPS-A overexpression (OE) Arabidopsis transgenics are supplemented with either ACC, the ethylene precursor, or AgNO3, the ethylene signaling inhibitor indicated that MIPS regulates the photosynthetic efficiency and chlorophyll content via the ethylene signaling pathway under control and thermal stress. Expression analysis of essential genes involved in the ethylene biosynthetic and signaling pathway corroborated.

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Funding

NS received a UGC fellowship from the University Grant Commission, Government of India. PK received financial support from the Department of Biotechnology, Government of India (BT/PR8406/AGIII/103/879/2013), and the JC Bose fellowship award, Science and Engineering Research Board, Government of India.

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  1. Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Naveen Sharma, Bhavya Shree & Paramjit Khurana

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  1. Naveen Sharma
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  2. Bhavya Shree
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  3. Paramjit Khurana
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Correspondence to Paramjit Khurana.

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Sharma, N., Shree, B. & Khurana, P. Myo-inositol phosphate synthase improves heat stress tolerance by ethylene-mediated modulation of chlorophyll content and photosynthetic efficiency. Protoplasma 260, 1097–1107 (2023). https://doi.org/10.1007/s00709-022-01835-1

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