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Comparative analysis of transcriptome in two wheat genotypes with contrasting levels of drought tolerance

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Abstract

Drought tolerance is a complex trait that is governed by multiple genes. The study presents differential transcriptome analysis between drought-tolerant (Triticum aestivum Cv. C306) and drought-sensitive (Triticum aestivum Cv. WL711) genotypes, using Affymetrix GeneChip® Wheat Genome Array. Both genotypes exhibited diverse global transcriptional responses under control and drought conditions. Pathway analysis suggested significant induction or repression of genes involved in secondary metabolism, nucleic acid synthesis, protein synthesis, and transport in C306, as compared to WL711. Significant up- and downregulation of transcripts for enzymes, hormone metabolism, and stress response pathways were observed in C306 under drought. The elevated expression of plasma membrane intrinsic protein 1 and downregulation of late embryogenesis abundant in the leaf tissues could play an important role in delayed wilting in C306. The other regulatory genes such as MT, FT, AP2, SKP1, ABA2, ARF6, WRKY6, AOS, and LOX2 are involved in defense response in C306 genotype. Additionally, transcripts with unknown functions were identified as differentially expressed, which could participate in drought responses.

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Acknowledgments

JK and SG acknowledge CSIR for fellowship.

Data access

The microarray raw data (.CEL files) have been deposited at NCBI in the Gene Expression Omnibus (GEO) database under the accession number: GSE87325.

Funding

Authors acknowledge the Department of Biotechnology (DBT), Govt. of India for the financial support.

Author information

Author notes

  1. Jitendra Kumar and Samatha Gunapati contributed equally to this work

Authors and Affiliations

  1. National Agri-Food Biotechnology Institute, Mohali, India

    Jitendra Kumar & Sudhir P. Singh

  2. USDA-ARS Cereal Disease Laboratory, St. Paul, MN, USA

    Jitendra Kumar & Shahryar F. Kianian

  3. Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN, USA

    Samatha Gunapati

  4. Center of Innovative and Applied Bioprocessing, Mohali, India

    Sudhir P. Singh

Authors
  1. Jitendra Kumar
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  2. Samatha Gunapati
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  3. Shahryar F. Kianian
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  4. Sudhir P. Singh
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Contributions

SPS designed the study. JK performed the experiments. SG analyzed the data. JK, SG, and SPS prepared the manuscript. SFK helped in data analysis and critical reading of the manuscript. All authors approved this version of the manuscript.

Corresponding author

Correspondence to Sudhir P. Singh.

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Conflict of interest

The authors declare that they have no conflict of interest.

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Handling Editor: Bhumi Nath Tripathi

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Kumar, J., Gunapati, S., Kianian, S.F. et al. Comparative analysis of transcriptome in two wheat genotypes with contrasting levels of drought tolerance. Protoplasma 255, 1487–1504 (2018). https://doi.org/10.1007/s00709-018-1237-x

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  • DOI: https://doi.org/10.1007/s00709-018-1237-x

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