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Cyclosis-mediated transfer of H2O2 elicited by localized illumination of Chara cells and its relevance to the formation of pH bands

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Abstract

Cytoplasmic streaming occurs in most plant cells and is vitally important for large cells as a means of long-distance intracellular transport of metabolites and messengers. In internodal cells of characean algae, cyclosis participates in formation of light-dependent patterns of surface pH and photosynthetic activity, but lateral transport of regulatory metabolites has not been visualized yet. Hydrogen peroxide, being a signaling molecule and a stress factor, is known to accumulate under excessive irradiance. This study was aimed to examine whether H2O2 produced in chloroplasts under high light conditions is released into streaming fluid and transported downstream by cytoplasmic flow. To this end, internodes of Chara corallina were loaded with the fluorogenic probe dihydrodichlorofluorescein diacetate and illuminated locally by a narrow light beam through a thin optic fiber. Fluorescence of dihydrodichlorofluorescein (DCF), produced upon oxidation of the probe by H2O2, was measured within and around the illuminated cell region. In cells exhibiting active streaming, H2O2 first accumulated in the illuminated region and then entered into the streaming cytoplasm, giving rise to the expansion of DCF fluorescence downstream of the illuminated area. Inhibition of cyclosis by cytochalasin B prevented the spreading of DCF fluorescence along the internode. The results suggest that H2O2 released from chloroplasts under high light is transported along the cell with the cytoplasmic flow. It is proposed that the shift of cytoplasmic redox poise and light-induced elevation of cytoplasmic pH facilitate the opening of H+/OH−-permeable channels in the plasma membrane.

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Acknowledgments

The authors would like to acknowledge Prof. Ralf Stannarius (Magdeburg University) for fruitful discussions and Chrisitan Köhler for participating in the experiments. This work was supported by Otto-von-Guericke-Universität Magdeburg and by the Russian Foundation for Basic Research.

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Authors and Affiliations

  1. Institute of Experimental Physics, Otto-von-Guericke-Universität Magdeburg, Universitätsplatz 2, 39016, Magdeburg, Germany

    Alexey Eremin & Marcus J. B. Hauser

  2. Faculty of Biology, Lomonosov Moscow State University, 119992, Moscow, Russia

    Alexander Bulychev

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  1. Alexey Eremin
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  2. Alexander Bulychev
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  3. Marcus J. B. Hauser
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Correspondence to Alexander Bulychev.

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Handling Editor: Liwen Jiang

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Eremin, A., Bulychev, A. & Hauser, M.J.B. Cyclosis-mediated transfer of H2O2 elicited by localized illumination of Chara cells and its relevance to the formation of pH bands. Protoplasma 250, 1339–1349 (2013). https://doi.org/10.1007/s00709-013-0517-8

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  • DOI: https://doi.org/10.1007/s00709-013-0517-8

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