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Cytological and molecular characterization of Vicia barbazitae Ten. & Guss.

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Abstract

Vicia barbazitae, a taxon belonging to section Vicia of subgenus Vicia, was recovered and analysed by cytological, karyological and molecular methods with the aim of both proposing a general characterisation of this species and studying the relationships among the species of section Vicia . Phylogenetic relationships among the species of the section Vicia and those of the sections Microcarinae, Wiggersia and Atossa were also analysed. Automated karyotype analysis has been determined after Feulgen’s reaction; chromosome banding was performed by sequence-specific fluorochrome staining. Fluorescent chromosome banding showed CMA+/DAPI- NOR-associated heterochromatin in the satellite pair. Karyomorphological parameters, based on symmetry indices, the dendrogram of linkage distance constructed on 37 chromosome parameters, as well as the molecular data based on internal transcribed spacer sequences provided information about phylogenetic position of this species inside the section Vicia and among the species belonging to the sections Microcarinae, Wiggersia, Atossa and Vicia. From our karyological and molecular results, it emerges that V. barbazitae can be considered a natural member of section Vicia.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Dipartimento di Biologia, Università di Pisa, Pisa, Italy

    M. Ruffini Castiglione & R. Cremonini

  2. Dipartimento A.F.N.E., Università della Tuscia, Viterbo, Italy

    M. Frediani & M. T. Gelati

  3. Stazione Sperimentale di Granicoltura per la Sicilia, Caltagirone, Italy

    G. Venora

  4. Istituto di Biologia e Biotecnologie Agrarie, CNR, Pisa, Italy

    L. Giorgetti

  5. Sezione di Biologia Vegetale, Dipartimento delle Scienze Biologiche, Università di Napoli Federico II, Napoli, Italy

    P. Caputo

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  1. M. Ruffini Castiglione
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  2. M. Frediani
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  3. M. T. Gelati
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  4. G. Venora
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Correspondence to M. Ruffini Castiglione.

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Ruffini Castiglione, M., Frediani, M., Gelati, M.T. et al. Cytological and molecular characterization of Vicia barbazitae Ten. & Guss.. Protoplasma 249, 779–788 (2012). https://doi.org/10.1007/s00709-011-0323-0

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  • DOI: https://doi.org/10.1007/s00709-011-0323-0

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