Skip to main content
SpringerLink
Log in

Lectin binding in Cryptomonas and Chroomonas (Cryptophyceae)

  • Original Article
  • Published:
Protoplasma Aims and scope Submit manuscript

Abstract

The cell envelopes of Cryptomonas and Chroomonas exhibited significant fluorescence using FITC-labelled concanavalin A and wheat germ agglutinin when the cells were fixed prior to lectin binding. The periplast became intensely labelled in Chroomonas whereas Cryptomonas showed fluorescing granula in its gullet/furrow region and on the cell surface. Lectin labelling followed by fixation showed only label of periplast remnants of lysed cells and of the flagella of Chroomonas. Isolated periplasts of Cryptomonas and Chroomonas were intensively labelled with both concanavalin A and wheat germ agglutinin. Glycostaining of gels, onto which total cell protein extracts were loaded, showed a glycoprotein of high molecular weight for Cryptomonas and Chroomonas and an additional glycoprotein for Cryptomonas species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

BSA:

Bovine serum albumin

chl:

Chlorophyll

CLSM:

Confocal laser scanning microscopy

ConA:

Concanavalin A

FITC:

Fluorescein isothiocyanate

IPC:

Inner periplast component

PAGE:

Polyacrylamide gel electrophoresis

SDS:

Sodium dodecyl sulphate

SPC:

Surface periplast component

WGA:

Wheat germ agglutinin

References

  • Bathke L, Rhiel E, Krumbein WE, Marquardt J (1999) Biochemical and immunochemical investigations on the light-harvesting system of the cryptophyte Rhodomonas sp.: Evidence for a photosystem I specific antenna. Plant Biology 1:516–523

    Article  CAS  Google Scholar 

  • Brett SJ, Perasso L, Wetherbee R (1994) Structure and development of the cryptomonad periplast: a review. Protoplasma 181:106–122

    Article  Google Scholar 

  • Dodge JD (1973) The fine structure of algal cells. Academic Press, London, New York

    Google Scholar 

  • Fujimoto K (1997) SDS-digested freeze-fracture replica labelling electron microscopy to study the two-dimensional distribution of integral membrane proteins and phospholipids in biomembranes: practical procedure, interpretation and application. Histochem Cell Biol 107:87–96

    Article  PubMed  CAS  Google Scholar 

  • Gantt E (1971) Micromorphology of the periplast of Chroomonas sp. (Cryptophyceae). J Phycol 7:177–184

    Google Scholar 

  • Gantt E, Edwards MR, Provasoli L (1971) Chloroplast structure of the Cryptophyceae. Evidence for phycobiliproteins within intrathylakoidal spaces. J Cell Biol 48:280–290

    Article  PubMed  CAS  Google Scholar 

  • Giroldo D, Vieira AAH (2002) An extracellular sulphated fucose-rich polysaccharide produced by a tropical strain of Cryptomonas obovata (Cryptophyceae). J Applied Phycol 14:185–191

    Article  CAS  Google Scholar 

  • Hausmann K (1978) Extrusive organelles in protists. Int Rev Cytol 52:197–276

    Article  PubMed  CAS  Google Scholar 

  • Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, c 1 and c 2 in higher plants, algae and natural phytoplankton. Biochem Physiol Pflanzen 167:191–194

    CAS  Google Scholar 

  • Kies L (1967) Oogamie bei Eremosphaera viridis De Bary. Flora, Abt B 157:1–12

    Google Scholar 

  • Kugrens P, Lee RE, Corliss JO (1994) Ultrastructure, biogenesis, and functions of extrusive organelles in selected non-ciliate protists. Protoplasma 181:164–190

    Article  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during assembly of head of bacteriophage T7. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  • Martin-Cereceda M, Roberts EC, Wootton EC, Bonaccorso E, Dyal P, Guinea A, Rogers D, Wright CJ, Novarino G (2010) Morphology, ultrastructure, and small subunit rDNA phylogeny of the marine heterotrophic flagellate Goniomonas aff. amphinema. J Eukaryot Microbiol 57(2):159–170

    Article  PubMed  CAS  Google Scholar 

  • Møller HJ, Poulsen JH (2002) Staining of glycoproteins/proteoglycans in SDS-Gels. In: Walker J (ed) The Protein Protocols Handbook, 2nd edn. Humana Press, Totowa, pp 773–777

    Chapter  Google Scholar 

  • Morrall S, Greenwood AD (1980) A comparison of the periodic substructure of the trichocysts of the Cryptophyceae and Prasinophyceae. Biosystems 12:71–83

    Article  PubMed  CAS  Google Scholar 

  • Novarino G (2003) A companion to the identification of cryptomonad flagellates (Cryptophyceae = Cryptomonadea). Hydrobiologia 502:225–270

    Article  Google Scholar 

  • Perasso L, Ludwig M, Wetherbee R (1997) The surface periplast component of the protist Komma caudata (Cryptophyceae) self-assembles from a secreted high-molecular-mass polypeptide. Protoplasma 200:186–197

    Article  CAS  Google Scholar 

  • Rhiel E, Westermann M (2011) Isolation, purification and some ultrastructural details of discharged ejectisomes of cryptophytes. Protoplasma. doi:10.1007/s00709-011-0267-4

  • Roberts EC, Zubkov MV, Martin-Cereceda M, Novarino G, Wootton EC (2006) Cell surface lectin-binding glycoconjugates on marine planktonic protists. FEMS Microbiol Lett 265:202–207

    Article  PubMed  CAS  Google Scholar 

  • Smith E, Roberts K, Hutchings A, Galfre G (1984) Monoclonal antibodies to the major structural glycoprotein of the Chlamydomonas cell wall. Planta 161:330–338

    Article  CAS  Google Scholar 

  • von Sengbusch P, Müller U (1983) Distribution of glycoconjugates at algal cell surfaces as monitored by FITC-conjugated lectins. Studies on selected species from Cyanophyta, Pyrrophyta, Raphidophyta, Euglenophyta, Chromophyta, and Chlorophyta. Protoplasma 114:103–113

    Article  Google Scholar 

  • von Stosch HA (1973) Observations on vegetative reproduction and sexual life cycles of two freshwater dinoflagellates. Gymnodinium pseudopalustre Schiller and Woloszyskia apiculata sp. nov. Brit Phycol J 8:105–134

    Article  Google Scholar 

Download references

Acknowledgements

The authors express their gratitude to Ms. Silke Ammermann for excellent technical assistance and Dr. PC Frost (Trent University, Peterborough, Canada) for his proof reading of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Planktologie, ICBM, Carl-von-Ossietzky-Universität Oldenburg, P.O.B. 2503, 26129, Oldenburg, Germany

    Erhard Rhiel

  2. Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359, Bremen, Germany

    Jörg Brock

Authors
  1. Erhard Rhiel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jörg Brock
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Erhard Rhiel.

Additional information

Handling Editor: Reimer Stick

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rhiel, E., Brock, J. Lectin binding in Cryptomonas and Chroomonas (Cryptophyceae). Protoplasma 249, 759–768 (2012). https://doi.org/10.1007/s00709-011-0319-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00709-011-0319-9

Keywords

Search

Navigation