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Pectin dynamic and distribution of exchangeable Ca2+ in Haemanthus albiflos hollow style during pollen–pistil interactions

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Abstract

In this report, the localization and spatial distribution of two categories of pectin, high and low methylesterified, on the background of dynamic in loosely bound calcium (Ca2+) in Haemanthus hollow style were studied before and after pollination. In the style transmitting tract of unpollinated pistil, mainly high-methylesterified pectins were present, both in the transmitting tract epidermis and in the style canal. After pollination, an increase in the level of two investigated categories of pectin was observed, but the amount of high-methylesterified one in each period of time analyzed was permanently higher. Locally, in the regions of the style canal penetrated by pollen tubes, process of pectin de-esterification was initiated. However, pollination caused an increase of loosely bound Ca2+ level in the style transmitting tract, this process appears to be not linked with pectin de-esterification and possible Ca2+ release after the lysis of Ca2+ cross-linked de-esterified pectin. Instead, it seems to be based on Ca2+ exocytosis from the transmitting tract epidermis cells providing a source of Ca2+ for pollen tubes growing in Haemanthus hollow style.

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Abbreviations

BSA:

bovine serum albumin

Ca2+ :

calcium

CRT:

calreticulin

ER:

endoplasmic reticulum

ecm:

extracellular matrix

HGs:

homogalactouronan

MAb JIM7:

monoclonal antibody against high-methylesterified homogalacturonic acid

MAb JIM5:

monoclonal antibody against low-methylesterified homogalacturonic acid

PBS:

phosphate-buffered saline

ppts:

Ca2+ precipitates

stt:

style transmitting tract

tte:

transmitting tract epidermis

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Acknowledgments

The authors thank Dr. JP Knox, Centre for Plant Biochemistry and Biotechnology, University of Leeds, UK for JIM5 and JIM7 antibodies, and Michał Świdziński for technical assistance. This project was supported by the Ministry of Science and Higher Education, grants 3 P04C 051 23 and N303 023 32/1034.

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Authors and Affiliations

  1. Laboratory of Developmental Biology, Faculty of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100, Toruń, Poland

    Marta Lenartowska

  2. Department of Cell Biology, Faculty of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100, Toruń, Poland

    Elżbieta Bednarska

  3. Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland

    Magdalena Krzesłowska

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  1. Marta Lenartowska
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  2. Magdalena Krzesłowska
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  3. Elżbieta Bednarska
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Correspondence to Marta Lenartowska.

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Handling Editor: Benedikt Kost

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Lenartowska, M., Krzesłowska, M. & Bednarska, E. Pectin dynamic and distribution of exchangeable Ca2+ in Haemanthus albiflos hollow style during pollen–pistil interactions. Protoplasma 248, 695–705 (2011). https://doi.org/10.1007/s00709-010-0231-8

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