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NaCl effect on the distribution of wall ingrowth polymers and arabinogalactan proteins in type A transfer cells of Medicago sativa Gabès leaves

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Abstract

We studied the distribution of wall ingrowth (WI) polymers by probing thin sections of companion cells specialized as transfer cells in minor veins of Medicago sativa cv Gabès blade with affinity probes and antibodies specific to polysaccharides and glycoproteins. The wall polymers in the controls were similar in WIs and in the primary wall but differently distributed. The extent of labeling in these papillate WIs differed for JIM5 and JIM7 homogalacturonans but was in the same range for LM5 and LM6 rhamnogalacturonans and xyloglucans. These data show that WI enhancement probably requires arabinogalactan proteins (JIM8) mainly localized on the outer part of the primary wall and WIs. By comparison, NaCl-treated plants exhibited cell wall polysaccharide modifications indicating (1) an increase in unesterified homogalacturonans (JIM5), probably implicated in Na+ binding and/or polysaccharide network interaction for limiting turgor variations in mesophyll cells; (2) enhancement of the xyloglucan network with an accumulation of fucosylated xyloglucans (CCRC-M1) known to increase the capacity of cellulose binding; and (3) specific recognition of JIM8 arabinogalactan proteins that could participate in both wall enlargement and cohesion by increasing the number of molecular interactions with the other polymers. In conclusion, the cell wall polysaccharide distribution in enlarged WIs might (1) participate in wall resistance to sequestration of Na+, allowing a better control of hydric homeostasis in mesophyll cells to maintain metabolic activity in source leaves, and (2) maintain tolerance of M. sativa to NaCl.

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Abbreviations

Mab:

Monoclonal antibody

PW:

Primary wall

type A TC:

Companion cell transfer cell

WI:

Wall ingrowth

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Acknowledgements

This work was supported by the Universities of Limoges, France (Faculté Sciences et Techniques, LCSN), Poitiers, France (FRE 3091 CNRS and Service Interdisciplinaire de Microscopie et d'Imagerie Scientifique), and Bizerte, Tunisia. The authors are grateful to V Ameteau, M Lallemand, and JM Perault for skillful technical assistance.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Département des Sciences de la Vie, Faculté des Sciences de Bizerte, 7021, Zarzouna, Bizerte, Tunisia

    Néziha Boughanmi

  2. Université Poitiers, Campus Sciences, FRE 3091CNRS, Bâtiment Botanique 40, Avenue Recteur Pineau, 86022, Poitiers, France

    Florence Thibault & Pierrette Fleurat-Lessard

  3. Université Poitiers, Campus Sciences, SIMIS, IBMIG, 40, Avenue Recteur Pineau, 86022, Poitiers, France

    Emile Béré

  4. Faculté des Sciences et Techniques, LCSN, Groupe de Glycobiologie Forestière, 123 Avenue Albert Thomas, 87060, Limoges, France

    Raphael Decou, Guy Costa & Sabine Lhernould

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  1. Néziha Boughanmi
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Correspondence to Sabine Lhernould.

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Boughanmi, N., Thibault, F., Decou, R. et al. NaCl effect on the distribution of wall ingrowth polymers and arabinogalactan proteins in type A transfer cells of Medicago sativa Gabès leaves. Protoplasma 242, 69–80 (2010). https://doi.org/10.1007/s00709-010-0125-9

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