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Formation of archegonium chamber is associated with nucellar-cell programmed cell death in Ginkgo biloba

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Summary.

The archegonium chamber in Ginkgo biloba L. is a pathway for spermatozoids swimming towards the archegonium for fertilization. The objective of this study was to elucidate the mechanism of archegonium chamber formation. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and DNA ladder demonstrated that the nucellar cell death, coordinated with the archegonium chamber formation, was a process of programmed cell death. Cytochemical localization of Ca2+ in these nucellar cells was determined by means of in situ precipitation with potassium pyroantimonate and electron microscopic visualization, in order to study the relation between Ca2+ and programmed cell death. The results showed an early uptake of the mitochondrial calcium particles in the nucellar cells undergoing programmed cell death. Together with other dynamic changes in Ca2+ subcellular distribution, this indicates that Ca2+ may play a role in the regulation of mitochondria-mediated programmed events in the nucellar cells.

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Authors and Affiliations

  1. School of Pharmaceutical Sciences, Peking University, Beijing

    Da-Hui Li

  2. College of Life Sciences, Peking University, Beijing

    Xiong Yang & Ke-Ming Cui

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  1. Da-Hui Li
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  2. Xiong Yang
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  3. Ke-Ming Cui
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Correspondence to Da-Hui Li.

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Correspondence and reprints: School of Pharmaceutical Sciences, Peking University, Beijing 100083, People’s Republic of China.

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Li, DH., Yang, X. & Cui, KM. Formation of archegonium chamber is associated with nucellar-cell programmed cell death in Ginkgo biloba . Protoplasma 231, 173–181 (2007). https://doi.org/10.1007/s00709-007-0257-8

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  • DOI: https://doi.org/10.1007/s00709-007-0257-8

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