Abstract
Monoclonal conjugates using radionuclides are widely used in the diagnosis or therapy of cancer. A number of ligand molecules per protein molecule (L/P ratio) are a crucial factor affecting the monoclonal conjugate efficacy and safety. For proper and accurate dosing of monoclonal conjugates, it is necessary to establish the L/P ratio before they are applied. The aim of this work was the development of a simple, efficient, non-radioactive, and low-cost spectrophotometric method for L/P ratio measurement. The method is based on transchelation reaction between the monoclonal conjugate and yttrium(III)–methylthymol blue complex followed at wavelength of 590 nm. The method operates in the L/P ratio range of 0.32–8.00 and the results are satisfactorily comparable to the conventional mass spectrometric method.
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Acknowledgements
The authors are grateful to Dr. Simon Cihelník for the helpful discussion on the beginning of the project. The financial support by Ministry of Industry and Trade of the Czech Republic (Project FT-TA2/081) and by projects SVV and Progress Q46 of Charles University is gratefully acknowledged.
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Nesměrák, K., Třmínková-Němcová, P., Drož, L. et al. Spectrophotometric determination of ligand–protein ratio in B2M3–DTPA monoclonal conjugates based on yttrium–methylthymol blue complex. Monatsh Chem 149, 1653–1657 (2018). https://doi.org/10.1007/s00706-018-2248-1
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DOI: https://doi.org/10.1007/s00706-018-2248-1