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Spectrometric methods for determination of ligand–protein ratio in monoclonal conjugates: a mini review

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Abstract

An overview is provided of spectrometric methods for determination of the number of ligand molecules per protein molecule in monoclonal conjugates used in radiodiagnostics or radiotherapy. Individual methods (UV/Vis, fluorescence, and infrared spectrometry) are discussed and examples of their application described.

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Acknowledgments

The authors are grateful for the financial support by the grant project SVV of Charles University in Prague.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic

    Karel Nesměrák & Pavlína Třmínková

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  1. Karel Nesměrák
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  2. Pavlína Třmínková
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Correspondence to Karel Nesměrák.

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Nesměrák, K., Třmínková, P. Spectrometric methods for determination of ligand–protein ratio in monoclonal conjugates: a mini review. Monatsh Chem 147, 1339–1342 (2016). https://doi.org/10.1007/s00706-016-1747-1

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  • DOI: https://doi.org/10.1007/s00706-016-1747-1

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