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Evaluation of an ion source with a tubular nebulizer for microflow atmospheric pressure chemical ionization

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Abstract

An atmospheric pressure chemical ionization (APCI) source designed for microliter-per-minute flow rates (0.5–10.0 mm3/min) was constructed. A simple resistively heated 1/8 in. OD tube-based nebulizer, together with a corona-discharge electrode was positioned in front of the entrance capillary of a mass spectrometer. Working parameters of the ion source were optimized, and its performance was evaluated. The limit of detection for directly infused acridine was found to be lower for micro-APCI (10 fmol/s) than for conventional APCI (170 fmol/s), and the linear dynamic range was significantly wider for the micro-APCI source. The micro-APCI and conventional APCI sources provided similar, but not identical mass spectra. The micro-APCI source was used as a detector for high-performance liquid chromatography at 10 mm3/min; the limit of detection for acridine was 690 fmol, which was about ten times lower value than in conventional HPLC.

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Acknowledgements

Financial support from the Czech Science Foundation (16-01639S) and Charles University in Prague (Project SVV260440) is hereby acknowledged with appreciation. The authors wish to thank Dr. Jindřich Houžvička (Crytur Ltd.) for providing them with the corundum tube.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Sciences, Charles University in Prague, Hlavova 2030/8, Prague 2, Czech Republic

    Timotej Strmeň & Josef Cvačka

  2. Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo nám. 2, Prague 6, Czech Republic

    Timotej Strmeň, Vladimír Vrkoslav, Ondřej Pačes & Josef Cvačka

Authors
  1. Timotej Strmeň
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  2. Vladimír Vrkoslav
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  3. Ondřej Pačes
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  4. Josef Cvačka
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Corresponding author

Correspondence to Josef Cvačka.

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Strmeň, T., Vrkoslav, V., Pačes, O. et al. Evaluation of an ion source with a tubular nebulizer for microflow atmospheric pressure chemical ionization. Monatsh Chem 149, 987–994 (2018). https://doi.org/10.1007/s00706-018-2172-4

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  • DOI: https://doi.org/10.1007/s00706-018-2172-4

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