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Enhanced Pb2+ biosorption by recombinant Saccharomyces cerevisiae expressing human metallothionein

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Abstract

Recombinant Saccharomyces cerevisiae expressing human hepatic metallothionein (SC-mt) was constructed for biosorption of lead(II). The gene sequence of mt was modified for codon preference of S. cerevisiae (SC) and synthesized using a chemical method. The influences of different experimental parameters such as solution pH, initial concentration, contact time, and temperature on adsorption were investigated. SC and SC-mt showed the highest Pb(II) sorption capacity at initial pH of 5.0 and contact time of 30 min. Adsorption on SC-mt was more favorable than on SC; in addition, the saturated monolayer sorption capacity increased from 80.00 to 181.82 mg/g at 298 K. Adsorption kinetics was better described by a pseudo-second-order model, and the adsorption process could be well described by the Langmuir isotherm. Thermodynamic parameters of SC-mt, namely ΔG°(298 K), ΔH°, and ΔS°, were determined to be −17.91 and 12.13 kJ mol−1, and 100.81 J mol−1 K−1, respectively, demonstrating that the sorption process of SC-mt towards Pb(II) was feasible, spontaneous, and endothermic in nature. The results indicate that the recombinant S. cerevisiae SC-mt should be useful for enhancement of the effectiveness of lead biosorption in practice.

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References

  1. Das N, Vimala R, Karthika P (2008) India J Biotechnol 71:59

    Google Scholar 

  2. Park D, Yun YS, Park JM (2010) Biotechnol Bioproc E 15:86

    Article  CAS  Google Scholar 

  3. Wang J, Chen C (2009) Biotechnol Adv 27:195

    Article  Google Scholar 

  4. Lin Z, Ye Y, Li Q, Xu Z, Wang M (2011) BMC Biotechnol 11:98

    Article  CAS  Google Scholar 

  5. Blériot C, Effantin G, Lagarde F, Mandrand-Berthelot MA, Rodrigue A (2011) J Bacteriol 193:3785

    Article  Google Scholar 

  6. Malik A (2004) Environ Int 30:261

    Article  CAS  Google Scholar 

  7. Gadd GM (2010) Microbiology 156:609

    Article  CAS  Google Scholar 

  8. Chen XC, Wang YP, Lin Q, Shi JY, Wu WX, Chen YX (2005) Colloids Surf B Biointerfaces 46:101

    Article  CAS  Google Scholar 

  9. Ghorbani S, Tabandeh F, Yakhchali B, Mehrnia MR (2011) Biotechnol Bioproc E 16:1019

    Article  CAS  Google Scholar 

  10. Kiyono M, Pan-Hou H (2006) J Health Sci 52:199

    Article  CAS  Google Scholar 

  11. Chen JP, Lin YS (2007) J Chin Inst Chem Eng, 38:235

    Article  CAS  Google Scholar 

  12. Su YJ, Lin JQ, Hao DH (2009) Biotechnol Bioproc E 14:565

    Article  CAS  Google Scholar 

  13. Wang J, Chen C (2006) Biotechnol Adv 24:427

    Article  CAS  Google Scholar 

  14. Zhang Y, Fan C, Meng Q, Diao Z, Dong L, Peng X, Ma S, Zhou Q (2009) Bull Environ Contam Toxicol 83:708

    Article  CAS  Google Scholar 

  15. Peng Q, Liu Y, Zeng G, Xu W, Yang C, Zhang J (2010) J Hazard Mater 177:676

    Article  CAS  Google Scholar 

  16. Wu Y, Wen Y, Zhou J, Dai Q, Wu Y (2012) Environ Sci Pollut Res 19:3371

    Article  CAS  Google Scholar 

  17. Dhankhar R, Hooda A, Solanki R, Sainger PA (2011) Int J Eng Sci Technol 3:5397

    Google Scholar 

  18. Kumar Y, King P, Prasad V (2006) Chem Eng J 124:63

    Article  CAS  Google Scholar 

  19. Pimentel PM, González G, Melo MFA, Melo DMA, Silvajr C, Assunção ALC (2007) Sep Purif Technol 56:348

    Article  CAS  Google Scholar 

  20. Anirudhan TS, Rijith S, Tharun AR (2010) Colloids Surf A 368:13

    Article  CAS  Google Scholar 

  21. Donat RJ (2009) Chem Thermodyn 41:829

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We are grateful to the National Science Foundation of China (Grant No. 21107014 and 21201033), Youth Science Foundation of Education Department of Jiangxi Province (Grant No. GJJ11140), National Science Foundation of China (Grant No. 21267001), and Fundamental Science on Radioactive Geology and Exploration Technology Laboratory Foundation (Grant No. REGT1217) for financial support.

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Authors and Affiliations

  1. Fundamental Science on Radioactive Geology and Exploration Technology Laboratory, East China Institute of Technology, Nanchang, China

    Min Li & Zhibin Zhang

  2. Laboratory Breeding Base of Nuclear Resources and Environment, East China Institute of Technology, Nanchang, China

    Min Li

  3. College of Biological Science and Engineering, Beifang University of Nationalities, Yinchuan, China

    Jie Shang

  4. Faculty of Chemistry Biology and Material Sciences, East China Institute of Technology, Nanchang, China

    Bo Liang & Liangliang Yu

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  1. Min Li
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  2. Zhibin Zhang
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  3. Jie Shang
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  4. Bo Liang
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  5. Liangliang Yu
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Corresponding author

Correspondence to Zhibin Zhang.

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J. Shang has equally contributed to this article.

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Li, M., Zhang, Z., Shang, J. et al. Enhanced Pb2+ biosorption by recombinant Saccharomyces cerevisiae expressing human metallothionein. Monatsh Chem 145, 235–240 (2014). https://doi.org/10.1007/s00706-013-1016-5

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  • DOI: https://doi.org/10.1007/s00706-013-1016-5

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