Summary.
The fluorescence detected circular dichroism (FDCD) appearance of some exciton coupling compounds is reviewed by a newly developed ellipsoidal mirror system (FDCD465). The FDCD465 achieves both the complete elimination of the polarization artifact and a dramatic enhancement of detection sensitivity expanding the applicability of exciton coupling fluorophores. A variety of chromophores that possess large extinction coefficients and high fluorescence quantum yields are available for exciton coupled FDCD, regardless of the degree of fluorescence polarization. Different types of FDCD devices can regulate FDCD polarization artifacts and the photoselection effect, enabling effective surveys of FDCD potential.
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Nehira, T. Fluorescence Detected Circular Dichroism for Highly Sensitive Observation of Exciton Coupled CD. Monatshefte für Chemie 136, 477–487 (2005). https://doi.org/10.1007/s00706-004-0271-x
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DOI: https://doi.org/10.1007/s00706-004-0271-x