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Isolation and artificial production of atypical porcine pestivirus, using the swine-kidney-derived cell line SK-L

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Abstract

Congenital tremor (CT) in piglets was first reported in 1922, and although the causative pathogen was unknown for many years, atypical porcine pestivirus (APPV) was recently shown to be the cause. APPV is difficult to isolate, and there have been few reports of APPV isolated from field materials. Here, we successfully isolated infectious particles from a tonsillar emulsion from a CT-affected piglet using the established swine-kidney-derived cell line SK-L. In addition, we produced APPV artificially using these cells. Thus, SK-L cells are useful for both isolation and artificial production of APPV.

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Fig. 1
Fig. 2

Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

We would like to thank Dr. Yoshihiro Sakoda from Hokkaido University for providing the SK-L cells. We also thank the National Institute of Animal Health (Tsukuba, Japan) for providing the PPK-3F, CPK, and CPK-NS cells.

Funding

This work was supported by a JSPS KAKENHI Grant-in-Aid for Scientific Research B (No. 22H02504).

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Authors and Affiliations

  1. Laboratory of Microbiology and Infectious Diseases, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1, Kyonancho, Musashino, Tokyo, 180-8602, Japan

    Mai Shiokawa, Yui Morita, Makoto Haritani & Hiroshi Aoki

  2. School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, 252-5201, Japan

    Makoto Nagai

  3. Environmental Science for Sustainable Development, Graduate school of Agriculture and Life Science, The University of Tokyo, Tokyo, 113-8657, Japan

    Makoto Haritani

Authors
  1. Mai Shiokawa
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  2. Yui Morita
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  3. Makoto Nagai
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  4. Makoto Haritani
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  5. Hiroshi Aoki
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Contributions

Mai Shiokawa designed the study and performed the experiments shown in Figure 2. Yui Morita supported the experiments shown in Figure 1A-C. Makoto Nagai provided and prepared the tonsil samples. Makoto Haritani performed the experiment shown in Figure 1D. Hiroshi Aoki designed the study and performed the experiments shown in Figure 1A-D. All of the authors read and approved the final manuscript.

Corresponding author

Correspondence to Hiroshi Aoki.

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The authors declare that they have no competing interests.

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The authors confirm that the ethical policies of the journal, as noted in the journal's author guidelines, have been adhered to, and approval was received from the appropriate ethics review committee. No experimental animals were used for the reported work.

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Shiokawa, M., Morita, Y., Nagai, M. et al. Isolation and artificial production of atypical porcine pestivirus, using the swine-kidney-derived cell line SK-L. Arch Virol 168, 294 (2023). https://doi.org/10.1007/s00705-023-05919-y

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