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Molecular characterization of a novel cytorhabdovirus infecting Plumbago indica L.

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Abstract

In 2021, Plumbago indica plants with necrotic spots on their leaves were observed in Beijing, China. Through high-throughput sequencing, we discovered a putative novel member of the genus Cytorhabdovirus, which was provisionally named "plumbago necrotic spot-associated virus" (PNSaV). The full-length negative-sense single-stranded RNA genome of this virus is 13,180 nucleotides in length and contains eight putative open reading frames (ORFs), in the order 3′ leader-N-(P′)-P-P3-M-G-P6-L-5′ trailer. Phylogenetic analysis and pairwise comparisons suggested that PNSaV is most closely related to pastinaca cytorhabdovirus 1, with 59.2% nucleotide sequence identity in the complete genome and 56.4% amino acid sequence identity in the L protein. These findings suggest that PNSaV should be considered a new member of the genus Cytorhabdovirus.

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Data availability

The viral genome sequences were deposited in the GenBank database. The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was supported by the undergraduate research program of China Agricultural University.

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Author notes

  1. Co-first author: Zhangyao Nie and Xiuqi Zhang.

Authors and Affiliations

  1. Ministry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China

    Zhangyao Nie, Xiuqi Zhang, Yingxi Li, Zongying Zhang, Chenggui Han & Ying Wang

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  1. Zhangyao Nie
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  2. Xiuqi Zhang
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  3. Yingxi Li
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  4. Zongying Zhang
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  5. Chenggui Han
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were mainly performed by ZN and XZ. HTS data analysis was performed by YL, XZ, and ZN. The manuscript was written by ZN, XZ, and YW, assisted by CH and ZZ. All authors read and approved the manuscript.

Corresponding author

Correspondence to Ying Wang.

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The authors have no relevant financial or non-financial interests to disclose.

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Handling Editor: Ralf Georg Dietzgen.

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Nie, Z., Zhang, X., Li, Y. et al. Molecular characterization of a novel cytorhabdovirus infecting Plumbago indica L.. Arch Virol 168, 289 (2023). https://doi.org/10.1007/s00705-023-05911-6

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  • DOI: https://doi.org/10.1007/s00705-023-05911-6

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