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Complete genome sequence of patchouli chlorosis-associated cytorhabdovirus, a new cytorhabdovirus infecting patchouli plants in Brazil

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Abstract

A cytorhabdovirus, tentatively named “patchouli chlorosis-associated cytorhabdovirus” (PCaCV), was identified in a patchouli plant, using high-throughput sequencing, and its genome sequence was confirmed by Sanger sequencing. The PCaCV genome consists of 12,913 nucleotides and contains six open reading frames in the order 3'-N-P'-P-P3-M-(G)-L-5'. The glycoprotein gene was found to contain stop codons in the coding frame; hence, this gene is considered defective. PCaCV is most closely related to tomato yellow mottle-associated virus, sharing 61.1% nucleotide sequence identity in the complete genome and 73.9% amino acid sequence identity in the L protein. These data suggest that PCaCV should be considered a new member of the genus Cytorhabdovirus, and the binomial species name "Cytorhabdovirus patchoulii" is proposed.

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Acknowledgments

MK and TN are CNPq fellows. Conselho Nacional de Desenvolvimento Científico e Tecnológico.

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Authors and Affiliations

  1. Department of Plant Pathology, University of Brasilia, Brasília, Federal District, Brazil

    Caterynne Melo Kauffmann & Tatsuya Nagata

  2. Embrapa Eastern Amazon, Belém, Pará, Brazil

    Alessandra de Jesus Boari

  3. Department of Molecular Biology, University of Brasilia, Brasília, Federal District, Brazil

    João Marcos Fagundes Silva & Tatsuya Nagata

  4. Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil

    Rosana Blawid

Authors
  1. Caterynne Melo Kauffmann
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  2. Alessandra de Jesus Boari
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  3. João Marcos Fagundes Silva
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  4. Rosana Blawid
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  5. Tatsuya Nagata
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Correspondence to Tatsuya Nagata.

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Kauffmann, C.M., de Jesus Boari, A., Silva, J.M.F. et al. Complete genome sequence of patchouli chlorosis-associated cytorhabdovirus, a new cytorhabdovirus infecting patchouli plants in Brazil. Arch Virol 167, 2817–2820 (2022). https://doi.org/10.1007/s00705-022-05594-5

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  • DOI: https://doi.org/10.1007/s00705-022-05594-5

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