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Novel plant-associated genomoviruses from the Brazilian Cerrado biome

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Abstract

The discovery rate of new plant viruses has increased due to studies involving high-throughput sequencing (HTS), particularly for single-stranded DNA viruses of the family Genomoviridae. We carried out an HTS-based survey of genomoviruses in a wide range of native and exotic trees grown in the Brazilian Cerrado biome, and the complete genome sequences of two novel members of the family Genomoviridae from two distinct genera were determined. Specific primers were designed to detect these genomoviruses in individual samples. A new gemykolovirus (Tecoma stans associated gemykolovirus) was detected in Tecoma stans, and a new gemykibivirus (Ouratea duparquetiana associated gemykibivirus) was detected in Ouratea duparquetiana. A gemykrogvirus related to Gila monster associated gemykrogvirus (80% pairwise identity) was also detected in foliar samples of Trembleya parviflora. Our pilot study paves the way for a better characterization of this diverse collection of genomoviruses as well as their interactions with the associated tree species.

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Acknowledgments

The authors are grateful for financial support from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), Capes (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), FAP-DF (Fundação de Apoio à Pesquisa do DF), and Embrapa (Empresa Brasileira de Pesquisa Agropecuária). The authors would like to thank Mr. Antonio Francisco Costa (Embrapa Hortaliças) for his help with Sanger sequencing.

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Author notes

  1. Flávia Milene B. Nery and Josiane G. Batista contributed equally to this work.

Authors and Affiliations

  1. Departamento de Fitopatologia, Universidade de Brasília (UnB), Campus Universitário Darcy Ribeiro, Brasília, DF, 70910-900, Brazil

    Flávia Milene B. Nery, Josiane G. Batista, Felipe Fochat. S. Melo, Fernando L. Melo, Juliana Gabrielle I. Silva, Luciane de Nazaré A. Reis & Rita C. Pereira-Carvalho

  2. Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, 70770-017, Brazil

    Simone G. Ribeiro

  3. Embrapa Vegetable Crops (Hortaliças), National Center for Vegetable Crops Research (CNPH), Brasília, DF, 70275-970, Brazil

    Leonardo S. Boiteux

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  1. Flávia Milene B. Nery
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Contributions

Conceptualization, F.M.B.N., J.G.B., and R.C.P.C. Data curation, F.M.B.N., F.F.S.M., J.G.B., and R.C.P.C. Formal analysis, F.M.B.N., J.G.B., F.L.M., and R.C.P.C. Funding acquisition, F.L.M, S.G.R, L.S.B., and R.C.P.C. Investigation, F.M.B.N., J.G.B., F.L.M., and R.C.P.C. Project administration, L.S.B. and R.C.P.C. Resources, R.O.R. and S.G.R. Supervision, R.C.P.C. Visualization, F.M.B.N., J.G.B., and R.C.P.C. Writing – original draft, F.M.B.N., J.G.B., M.E.N.F., L.S.B., and R.C.P.C. Writing – review and editing, F.M.B.N., J.G.I.S., L.N.A.R., J.G.B., F.F.S.M., F.L.M., M.E.N.F., L.S.B., S.G.R., R.O.R., and R.C.P.C.

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Correspondence to Rita C. Pereira-Carvalho.

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Communicated by Jesús Navas-Castillo

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Nery, F.M.B., Batista, J.G., Melo, F.F.S. et al. Novel plant-associated genomoviruses from the Brazilian Cerrado biome. Arch Virol 168, 286 (2023). https://doi.org/10.1007/s00705-023-05892-6

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