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Complete genomic sequence of an isolate of plant-associated genomovirus 12 (genus Gemycircularvirus) from open–field tomatoes in Brazil

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Abstract

Using a next–generation sequencing (NGS) approach, we identified a gemycircularvirus (Genomoviridae) in association with foliar samples of open–field tomatoes in Brazil. The viral ssDNA genome (2,189 nucleotides) was amplified and Sanger–sequenced. BLASTn and sequence demarcation tool (SDT) analyzes showed that the virus from tomato shares 99% identity with a virus tentatively named as plant–associated genomovirus 12 from Larrea tridentata (Zygophyllaceae). SDT analyzes of a wide array of Genomoviridae sequences indicated this virus as a member of the genus Gemycircularvirus. This the first report of a gemycircularvirus in natural association with a species of the Solanaceae.

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Acknowledgements

This research was supported by grants, scholarships and post–doc fellowships from Embrapa, FAP–DF, CAPES, and CNPq.

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Authors and Affiliations

  1. Departamento de Fitopatologia, Universidade de Brasília (UnB), Área de Virologia Vegetal, Brasília, DF, Brazil

    Luciane de Nazaré Almeida dos Reis, Josiane Goulart Batista, Flávia Milene Barros Nery & Rita de Cássia Pereira–Carvalho

  2. National Center for Vegetable Crops Research (CNPH), Embrapa Hortaliças, Brasília, DF, Brazil

    Leonardo Silva Boiteux & Maria Esther de Noronha Fonseca

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  1. Luciane de Nazaré Almeida dos Reis
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  2. Leonardo Silva Boiteux
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  3. Maria Esther de Noronha Fonseca
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  4. Josiane Goulart Batista
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  5. Flávia Milene Barros Nery
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  6. Rita de Cássia Pereira–Carvalho
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Correspondence to Rita de Cássia Pereira–Carvalho.

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dos Reis, L.d., Boiteux, L.S., de Noronha Fonseca, M.E. et al. Complete genomic sequence of an isolate of plant-associated genomovirus 12 (genus Gemycircularvirus) from open–field tomatoes in Brazil. J Plant Pathol 104, 1129–1134 (2022). https://doi.org/10.1007/s42161-022-01151-w

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  • DOI: https://doi.org/10.1007/s42161-022-01151-w

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