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Complete genome sequence of a novel double-stranded RNA virus infecting the phytopathogenic fungus Rhizopus stolonifer

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Abstract

In this study, we report the complete genome sequence of a novel toti-like virus, tentatively named "Rhizopus stolonifer double-stranded RNA virus 1" (RsDSV1), identified from a phytopathogenic fungal agent of apple fruit rot disease, Rhizopus stolonifer strain A2-1. RsDSV1 has a double-stranded RNA genome. The complete RsDSV1 genome is 5178 nucleotides (nt) in length and contains two open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis based on RdRp and CP amino acid sequences revealed that RsDSV1 is closely related to unclassified members of the family Totiviridae. In stress-inducing Vogel’s minimal and sodium dodecyl sulfate-containing media, hyphal growth of A2-1 was suppressed, but the accumulation of RsDSV1 RNA increased, indicating that stresses promote RsDSV1 replication. To our knowledge, this is the first report of a mycovirus found in R. stolonifer.

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Acknowledgments

We thank the High-Performance Computing Center of Northwest A&F University for providing computing resources. This work was supported in part by the National Natural Science Foundation of China (31970163) and the Natural Science Foundation of Xinjiang Uygur Autonomous Region (2021D01D12) to LY. Sun and (31970159) to IB. Andika.

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China

    Hao Wang, Jingxian Luo, Ruoyin Dai, Kaleem Ullah Shah & Liying Sun

  2. College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China

    Ida Bagus Andika

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  1. Hao Wang
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  2. Jingxian Luo
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  3. Ruoyin Dai
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  4. Kaleem Ullah Shah
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  6. Liying Sun
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Correspondence to Liying Sun.

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Communicated by Massimo Turina

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Wang, H., Luo, J., Dai, R. et al. Complete genome sequence of a novel double-stranded RNA virus infecting the phytopathogenic fungus Rhizopus stolonifer. Arch Virol 168, 239 (2023). https://doi.org/10.1007/s00705-023-05869-5

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