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Molecular characterization of a novel amalgavirus infecting lilium spp. in China

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A Correction to this article was published on 18 July 2023

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Abstract

A novel plant virus with a double-stranded (ds) RNA genome was detected in Lilium spp. in China by high-throughput sequencing and tentatively named “lily amalgavirus 2” (LAV2). The genomic RNA of LAV2 is 3432 nucleotides (nt) in length and contains two open reading frames (ORFs) that putatively encode a ‘1 + 2’ fusion protein of 1053 amino acids (aa), generated by a ‘+1’ programmed ribosomal frameshift (PRF). ORF1 encodes a putative 386-aa protein of unknown function, and ORF2 overlaps ORF1 by 350 nt and encodes a putative 783-aa protein with conserved RNA-dependent RNA polymerase (RdRp) motifs. The ‘+1’ ribosomal frameshifting motif, UUU_CGN, which is highly conserved among amalgaviruses, is also found in LAV2. Sequence analysis showed that the complete genome shared 46.04%-51.59% nucleotide sequence identity with those of members of the genus Amalgavirus and had the most similarity (51.59% sequence identity) to lily amalgavirus 1 (accession no. OM782323). Phylogenetic analysis based on RdRp amino acid sequences showed that LAV2 clustered with members of the genus Amalgavirus. Overall, our data suggest that LAV2 is a new member of the genus Amalgavirus.

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Acknowledgements

This study was supported by the National Key Research and Development Program of China (2019YFD1001800).

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Authors and Affiliations

  1. Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China

    Zhihao Yuan, Zhenfeng Li, Yuexia Lu, Ni Hong, Guoping Wang & Li Cai

  2. National Citrus Engineering Research Center, Southwest University, 400712, Chongqing, China

    Mengji Cao

Authors
  1. Zhihao Yuan
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  2. Zhenfeng Li
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  4. Mengji Cao
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  5. Ni Hong
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Contributions

Z.H. Yuan and L. Cai designed the experiments and wrote the manuscript. Z.F. Li and Y.X. Lu contributed to sample preparation. M.J. Cao contributed to data analysis. H. Ni, G.P. Wang, and L. Cai contributed to critical revision of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Li Cai.

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This article does not contain any experiments with human participants or animals performed by any of the authors.

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Communicated by Massimo Turina

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Yuan, Z., Li, Z., Lu, Y. et al. Molecular characterization of a novel amalgavirus infecting lilium spp. in China. Arch Virol 168, 181 (2023). https://doi.org/10.1007/s00705-023-05806-6

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