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Molecular characterization of a novel deltaflexivirus infecting the edible fungus Pleurotus ostreatus

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Abstract

A novel positive single-stranded RNA virus, Pleurotus ostreatus deltaflexivirus 1 (PoDFV1), was isolated from the edible fungus Pleurotus ostreatus strain ZP6. The complete genome of PoDFV1 is 7706 nucleotides (nt) long and contains a short poly(A) tail. PoDFV1 was predicted to contain one large open reading frame (ORF1) and three small downstream ORFs (ORFs 2-4). ORF1 encodes a putative replication-associated polyprotein of 1979 amino acids (aa) containing three conserved domains – viral RNA methyltransferase (Mtr), viral RNA helicase (Hel), and RNA-dependent RNA polymerase (RdRp) – which are common to all deltaflexiviruses. ORFs 2-4 encode three small hypothetical proteins (15-20 kDa) without conserved domains or known biological functions. Sequence alignments and phylogenetic analysis suggested that PoDFV1 is a member of a new species in the genus Deltaflexivirus (family Deltaflexiviridae, order Tymovirales). To our knowledge, this is the first report of a deltaflexivirus infecting P. ostreatus.

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Data availability

The PoDFV1 genome sequence has been deposited in the GenBank database under accession number OQ295989.

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Funding

This research was supported by the National Natural Science Foundation of China (32202381) and the China Agriculture Research System of MOF and MARA (CARS-31).

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Authors and Affiliations

  1. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, 510642, China

    Junbo Xiao, Xin Wang, Ziru Zheng, Yugu Wu, Zhe Wang, Huaping Li & Pengfei Li

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  1. Junbo Xiao
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  2. Xin Wang
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  3. Ziru Zheng
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  4. Yugu Wu
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  5. Zhe Wang
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  7. Pengfei Li
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Correspondence to Huaping Li or Pengfei Li.

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Handling Editor: Nobuhiro Suzuki.

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Figure S1

A diagram showing the genomic organization of the other five viruses of the family Deltaflexiviridae: Alternaria tenuissima deltaflexivirus 1 (AtDFV1), Fusarium graminearum deltaflexivirus 1 (FgDFV1), Sclerotinia sclerotiorum deltaflexivirus 1 (SsDFV1), Sclerotinia sclerotiorum deltaflexivirus 2 (SsDFV2), Sclerotinia sclerotiorum deltaflexivirus 3 (SsDFV3). Open reading frames (ORFs) are represented by the box. Nucleotide numbers are indicated. Mtr, methyltransferase; Hel, helicase; RdRp, RNA dependent RNA polymerase (JPG 203 KB)

Figure S2

Multiple alignments of the three conserved domains of PoDFV1 and corresponding regions of other selected deltaflexiviruses. (A) the viral methyltransferase; (B) viral RNA helicase; (C) RNA-dependent RNA polymerase. Motif positions are indicated by lines above the sequences numbered from I to VI. Identical or conserved amino acid residues are shaded (JPG 1236 KB)

Supplementary file3 (DOCX 18 KB)

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Xiao, J., Wang, X., Zheng, Z. et al. Molecular characterization of a novel deltaflexivirus infecting the edible fungus Pleurotus ostreatus. Arch Virol 168, 162 (2023). https://doi.org/10.1007/s00705-023-05789-4

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