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Molecular characterization of a new gammapartitivirus isolated from the citrus-pathogenic fungus Penicillium digitatum

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Abstract

To date, partitiviruses, including gammapartitiviruses, have been extensively studied in various fungal hosts but have not been reported in Penicillium digitatum (also called green mold, the pathogenic fungus infecting citrus). In the present work, we isolated and molecularly characterized a double-stranded RNA (dsRNA) partitivirus from citrus green mold, which we have named “Penicillium digitatum gammapartitivirus 1” (PdGV1). The bisegmented genome of PdGV1 contains two dsRNA segments (dsRNA1 and dsRNA2) with a length of 1795 bp and 1622 bp, respectively. Each of the two genomic dsRNAs contains a single open reading frame encoding a putative RNA-dependent RNA polymerase (RdRp) and a coat protein (CP), respectively. Phylogenetic analysis based on RdRp and CP sequences showed that PdGV1 clustered with mycoviruses belonging to the genus Gammapartitivirus, family Partitiviridae, e.g., Penicillium stoloniferum virus S. The 5’- and 3’-untranslated regions (UTRs) of the PdGV1 genomic dsRNAs both contained unique conserved RNA motifs that have never been found in any other partitivirus. This is the first report of a new gammapartitivirus that infects the citrus-pathogenic fungus P. digitatum.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No.31371893 and 31071653), the Self-Determined Research Funds of CCNU from the Colleges’ Basic Research and Operation of MOE (No. CCNU17CG003), and the Open Funding from Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization (No. 2017BW03). We sincerely thank Dr. Huazhong Shi (Department of Chemistry and Biochemistry, Texas Tech University, USA) for English language editing.

Funding

This work was financially supported by the National Natural Science Foundations of China (No. 31371893 and 31071653), the Self-Determined Research Funds of CCNU from the Colleges’ Basic Research and Operation of MOE (No. CCNU17CG003), and the Open Funding from Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization (No. 2017BW03).

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Author notes

  1. Zhu Yang and Hui Geng contributed equally to this work.

Authors and Affiliations

  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, People’s Republic of China

    Zhu Yang, Hui Geng, Yongze Yuan, Menglan Wang, Jiali Mao, Tingfu Zhang, Yuhui Niu & Deli Liu

  2. College of Life Sciences, Huanggang Normal University, Huanggang, 438000, Hubei, People’s Republic of China

    Yongliang Zheng

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  1. Zhu Yang
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Correspondence to Yongze Yuan or Deli Liu.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Robert H.A. Coutts.

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Yang, Z., Geng, H., Zheng, Y. et al. Molecular characterization of a new gammapartitivirus isolated from the citrus-pathogenic fungus Penicillium digitatum. Arch Virol 163, 3185–3189 (2018). https://doi.org/10.1007/s00705-018-3951-2

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  • DOI: https://doi.org/10.1007/s00705-018-3951-2

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