Abstract
Birds carry a large number of viruses that may cause diseases in animals or humans. At present, information about the virome of zoo birds is limited. In this study, using viral metagenomics, we investigated the fecal virome of zoo birds collected from a zoo in Nanjing, Jiangsu Province, China. Three novel parvoviruses were obtained and characterized. The genomes of the three viruses are 5,909, 4,411, and 4,233 nt in length, respectively, and contain four or five ORFs. Phylogenetic analysis showed that these three novel parvoviruses clustered with other strains and formed three different clades. Pairwise comparison of NS1 amino acid sequences showed that Bir-01-1 shared 44.30-74.92% aa sequence identity with other parvoviruses belonging to the genus Aveparvovirus, while Bir-03-1 and Bir-04-1 shared less than 66.87% and 53.09% aa sequence identity, respectively, with other parvoviruses belonging to the genus Chaphamaparvovirus. Each of these three viruses was identified as a member of a novel species based on the species demarcation criteria for parvoviruses. These findings broaden our knowledge of the genetic diversity of parvoviruses and provide epidemiological data regarding potential outbreaks of parvovirus disease in birds.
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Funding
This work was supported by the National Key Research and Development Programs of China (No. 2022YFC2603801), the National Natural Science Foundation of China (No. 32102682), the Science Foundation of Higher Education of Jiangsu Province (No. 21KJB230006), and the Independent Project of Chengdu Research Base of Giant Panda Breeding (No. 2020CPB-C11).
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The research reported here did not involve experimentation with human participants or animals.
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Communicated by Ana Cristina Bratanich
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Yan Wang, Yijie Sun and Xin Li contributed equally to this work.
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Supplementary Material 1: Fig. S1:
Pairwise comparison of NS1 amino acid sequences of the three parvoviruses identified in this study with the representative strains of different genera of the family Parvoviridae. The pairwise comparison was conducted using SDTv1.2 software.
Supplementary Material 2: Fig. S2:
Phylogenetic analysis of three parvoviruses using the software MrBayes v3.2.7. (A) Phylogenetic tree constructed based on amino acid sequences of NS1. (B) Phylogenetic tree constructed based on amino acid sequences of VP1. The Markov chain was run for a maximum of one million generations, in which every 50 generations were sampled and the first 25% of the Markov chain Monte Carlo samples were discarded as burn-in.
Supplementary Material 3: Table. S1:
. Information of about the bird species and library included in the present study.
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Wang, Y., Sun, Y., Li, X. et al. Molecular detection and characterization of three novel parvoviruses belonging to two different subfamilies in zoo birds. Arch Virol 168, 163 (2023). https://doi.org/10.1007/s00705-023-05785-8
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DOI: https://doi.org/10.1007/s00705-023-05785-8