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Molecular characterization of a novel endornavirus isolated from Ophiostoma bicolor associated with bark beetles

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Abstract

Ophiostoma bicolor is a pathogenic fungus associated with bark beetles that can cause serious damage to host plants. In this study, a novel fungal virus, “Ophiostoma bicolor endornavirus 1” (ObEV1), was obtained from O. bicolor, and its complete genome sequence was determined. ObEV1 has a single-stranded positive-sense (+ ss) RNA genome of 10,119 nucleotides. Sequence annotation and comparison showed that the viral genome has a single large open reading frame (ORF) encoding a polyprotein of 362.48 kDa. The polyprotein contains seven conserved domains: RNA-dependent RNA polymerase (RdRp), viral RNA helicase 1 (VHel1), viral methyltransferase (VMet), DEAD-like helicase (DEXDc), gliding-GltJ (G1), large tegument protein UL36 (PHA), and YlqF-related-GTPase (Y). Sequence comparisons and phylogenetic analysis showed that ObEV1 is a novel mycovirus belonging to the genus Betaendornavirus of the family Endornaviridae. This is the first report of a mycovirus in the ophiostomatoid fungus O. bicolor.

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Acknowledgements

The authors are grateful to Dr. Huanting Liu (University of St. Andrews) and Wenyi Liu (Zhejiang A&F University, China) for help in improvement of the manuscript.

Funding

This research was supported by the National Key R&D Program of China (2017YFD0600102-7) and the Agricultural Science and Technology Fund of Shandong Province of China (Innovation Project of Forestry Science and Technology) Project (2019LY003-4).

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Authors and Affiliations

  1. Shandong Research Center for Forestry Harmful Biological Control Engineering and Technology, College of Plant Protection, Shandong Agricultural University, 271018, Tai’an, Shandong, People’s Republic of China

    Yuyao Zhu, Anna Lu, Chengming Yu & Huixiang Liu

  2. Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, 100091, Beijing, People’s Republic of China

    Zheng Wang & Quan Lu

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  1. Yuyao Zhu
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  2. Anna Lu
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  3. Zheng Wang
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Correspondence to Chengming Yu or Huixiang Liu.

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Zhu, Y., Lu, A., Wang, Z. et al. Molecular characterization of a novel endornavirus isolated from Ophiostoma bicolor associated with bark beetles. Arch Virol 167, 2839–2843 (2022). https://doi.org/10.1007/s00705-022-05613-5

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