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Molecular characterization of a novel mitovirus from Rhizoctonia solani AG-4 HGIII strain XMC-IF

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Abstract

The nucleotide sequence of a viral double-stranded RNA (dsRNA) from Rhizoctonia solani AG-4 HGIII strain XMC-IF (designated as "Rhizoctonia solani mitovirus 106", RsMV-106) was determined. The complete sequence was 2794 bp in length with a 57.50% A + U content and contained a large open reading frame (ORF) when the fungal mitochondrial genetic code was used. The ORF potentially encodes a 95.76-kDa protein containing a conserved domain of an RNA-dependent RNA polymerase (RdRp). BLASTp analysis revealed that the RdRp domain of RsMV-106 shared 47.52–73.24% sequence identity with those of viruses of the genus Duamitovirus and was most similar (73.24% identity) to that of Alternaria alternata mitovirus 1 (AaMV1). Phylogenetic analysis showed that RsMV-106 is a novel member of the genus Duamitovirus, family Mitoviridae. This is the first report of the full genome sequence of a mitovirus associated with R. solani AG-4 HGIII.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (no. 31960525, no. 31160352, and no. 31360423) and the National Natural Scientific Foundation of Yunnan (no. 202207AA110005).

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Authors and Affiliations

  1. State Key Laboratory for Protection and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, 650201, Kunming, Yunnan, China

    Aili Sun, Yang Sun, Li Luo, Lianjing Zhao, Chengyun Li & Genhua Yang

  2. Technology Department, Yunnan Agricultural University, 650201, Kunming, Yunnan, China

    Wenhan Dong

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  1. Aili Sun
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  2. Yang Sun
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  3. Li Luo
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  4. Lianjing Zhao
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  5. Chengyun Li
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  6. Genhua Yang
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  7. Wenhan Dong
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Correspondence to Genhua Yang or Wenhan Dong.

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Sun, A., Sun, Y., Luo, L. et al. Molecular characterization of a novel mitovirus from Rhizoctonia solani AG-4 HGIII strain XMC-IF. Arch Virol 167, 2821–2825 (2022). https://doi.org/10.1007/s00705-022-05599-0

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