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Transcription analysis of chicken embryo fibroblast cells infected with the recombinant avian leukosis virus isolate GX14FF03

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Abstract

Infection with recombinant avian leukosis virus (ALV) has previously been linked to malignancies and immunosuppression. However, the processes behind the unique pathophysiology of recombinant ALV are poorly understood. In this study, we analyzed gene expression patterns in chicken fibroblast cells (CEFs) infected with the recombinant ALV isolate GX14FF03 and used the RNA-seq technique to perform a complete analysis of the transcribed mRNAs. A total of 907 significant differentially expressed genes (SDEGs) were identified. Among these SDEGs, the most significantly upregulated gene was interleukin 8-like 1 (IL8L1), while the most significantly downregulated gene was fibroblast growth factor 16 (FGF16). The 907 SDGEs were highly enriched (p < 0.05) for 252 Gene Ontology (GO) terms, including 197 BP, 3 CC, and 52 MF. According to KEGG data analysis, SDEGs are implicated in eight significant pathways (p < 0.05). Furthermore, protein-protein interaction (PPI) network analysis revealed that IL8L1 interacts with 17 genes. These findings shed light on the molecular mechanisms involved in recombinant ALV infection by showing the mRNA expression profile in CEFs infected with GX14FF03 virus.

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Availability of data and materials

The datasets used and/or analyzed in the current study are available from the corresponding author upon reasonable request.

Abbreviations

ALV:

Avian leukosis virus

CEFs:

Chicken fibroblast cells

SDEGs:

Significant differentially expressed genes

IL8L1:

Interleukin 8-like 1

FGF16:

Fibroblast growth factor 16

GO:

Gene Ontology

DMEM:

Dulbecco’s modified Eagle medium

FBS:

Fetal bovine serum

TCID50 :

50% Tissue culture infectious dose

PPI:

Protein-protein interaction

qPCR:

Real-time quantitative PCR

BP:

Biological process

CC:

Cellular component

MF:

Molecular function

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Acknowledgements

The manuscript was kindly reviewed by Dr. Richard Roberts, Aurora, CO 80014, USA.

Funding

This work was supported by the Shandong Provincial Natural Science Foundation [ZR2019BC047], the Guangxi Special Funding on Science and Technology Research [AA17204057], the Guangxi Program for Modern Agricultural Industry Technical System Construction-Chicken Industry [nycytxgxcxtd-19-03], the Major Basic Program of Natural Science Foundation of Shandong Province, China [ZR2019ZD21], the Taishan Scholars Program of Shandong Province, China [ts20190955].

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Authors and Affiliations

  1. Institute of Microbe and Host Health, Linyi University, Linyi, 276000, China

    Peikun Wang

  2. Institute for Poultry Science and Health, Guangxi University, Nanning, 530004, China

    Qiuhong Li, Qiaomu Deng, Min Li & Ping Wei

  3. Animal Epidemic Disease Anticipatory Control Center, Lanshan District, Linyi, 276000, China

    Wangjing

Authors
  1. Peikun Wang
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  2. Qiuhong Li
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  4. Qiaomu Deng
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  5. Min Li
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  6. Ping Wei
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Contributions

Conceptualization: WPK. WJ conceived and designed the experiments. LQH collected the samples and data. WPK, LQH, DQM, and LM performed the experiments; WPK analyzed the data and wrote the manuscript. DQM and LM reviewed and edited the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Peikun Wang or Ping Wei.

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The authors declare no conflict of interest.

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No animal experiments were performed in this study.

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Wang, P., Li, Q., Wangjing et al. Transcription analysis of chicken embryo fibroblast cells infected with the recombinant avian leukosis virus isolate GX14FF03. Arch Virol 167, 2613–2621 (2022). https://doi.org/10.1007/s00705-022-05597-2

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  • DOI: https://doi.org/10.1007/s00705-022-05597-2

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