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Complete genome sequence of a putative novel cytorhabdovirus isolated from Rudbeckia sp.

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Abstract

Through high-throughput RNA sequencing, we discovered a putative new cytorhabdovirus in the seeds of Rudbeckia sp., which we have tentatively named “rudbeckia virus 1” (RudV1). Its complete 12,502-nt genomic sequence contains five open reading frames (ORFs): ORF1 (putative nucleocapsid protein, N), ORF2 (putative phosphoprotein, P), ORF3 (putative cell-to-cell movement protein, P3), ORF4 (putative matrix protein, M), and ORF5 (putative RNA-dependent RNA polymerase, L). BLASTp searches showed that ORF1, ORF3, ORF4, and ORF5 of RudV1 are most closely related to the corresponding proteins of Tagetes erecta virus 1 (a putative member of the genus Cytorhabdovirus) with 33.87% (88% query coverage), 55.98% (89% query coverage), 35.33% (94% query coverage), and 57.75% (98% query coverage) sequence identity at the amino acid level, respectively. Phylogenetic analysis and pairwise comparisons indicated that RudV1 is a novel member of the genus Cytorhabdovirus within the family Rhabdoviridae.

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Acknowledgements

This work was supported by a research fund (project no. B-1543086-2021-22-01) from the Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. We would like to thank Editage (www.editage.co.kr) for English language editing.

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Authors and Affiliations

  1. Plant Quarantine Technology Center, Department of Plant Quarantine, Animal and Plant Quarantine Agency, Gimcheon, 39660, Republic of Korea

    Da-Som Lee, Juhyun Kim, Minji Jun, Sanghyun Shin, Seong-Jin Lee & Seungmo Lim

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  1. Da-Som Lee
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  2. Juhyun Kim
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  3. Minji Jun
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  4. Sanghyun Shin
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  5. Seong-Jin Lee
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Contributions

Seungmo Lim and Seong-Jin Lee contributed to the study conception and design. Experiments, data collection, and analysis were performed by Da-Som Lee, Juhyun Kim, Minji Jun, Sanghyun Shin, and Seungmo Lim. Da-Som Lee and Seungmo Lim wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Seungmo Lim.

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Handling Editor: Ralf Georg Dietzgen.

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Lee, DS., Kim, J., Jun, M. et al. Complete genome sequence of a putative novel cytorhabdovirus isolated from Rudbeckia sp.. Arch Virol 167, 2381–2385 (2022). https://doi.org/10.1007/s00705-022-05556-x

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