Skip to main content
SpringerLink
Log in

Complete nucleotide sequence of a putative new cytorhabdovirus infecting lettuce

  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

The full-length nucleotide sequence of the genomic RNA of a new cytorhabdovirus infecting lettuce was determined. Six open reading frames were found in the antigenomic sequence of the 12,926-nt negative-sense viral RNA genome. The genomic organisation was similar to that of lettuce necrotic yellows virus (LNYV), the type member of the genus Cytorhabdovirus: 3′-N-P-3-M-G-L-5′, where N is the capsid protein gene, P the putative phosphoprotein gene, 3 a gene coding for a putative protein of unknown function, M the putative matrix protein gene, G the glycoprotein gene, and L the putative polymerase gene. Amino acid sequence comparison with the corresponding sequences of other rhabdoviruses revealed the closest relationship to LNYV, with identities ranging from 41% for the matrix proteins and 65% for the L polymerase proteins. These results indicate that this virus may be a member of a new cytorhabdovirus species, for which the name Lettuce yellow mottle virus (LYMoV) is proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • SF Altschul W Gish W Miller EW Myers DJ Lipman (1990) ArticleTitleBasic local alignment search tool J Mol Biol 215 403–410 Occurrence Handle2231712 Occurrence Handle1:CAS:528:DyaK3MXitVGmsA%3D%3D

    PubMed  CAS  Google Scholar 

  • SF Altschul TL Madden AA Schaffer J Zhang Z Zhang W Miller DJ Lipman (1997) ArticleTitleGapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res 25 3389–3402 Occurrence Handle9254694 Occurrence Handle10.1093/nar/25.17.3389 Occurrence Handle1:CAS:528:DyaK2sXlvFyhu7w%3D

    Article  PubMed  CAS  Google Scholar 

  • AK Banergee (1987) ArticleTitleTranscription and replication of rhabdoviruses Microbiol Rev 51 66–87

    Google Scholar 

  • D Blancard H Lot B Maisonneuve (2005) A colour atlas of diseases of lettuce and related salad crops Manson Publishing Iowa 319

    Google Scholar 

  • H Bourhy B Kissi N Tordo (1993) ArticleTitleMolecular diversity of the Lyssavirus genus Virology 194 70–81 Occurrence Handle8386891 Occurrence Handle10.1006/viro.1993.1236 Occurrence Handle1:CAS:528:DyaK3sXktVCmu7k%3D

    Article  PubMed  CAS  Google Scholar 

  • Callaghan B (2005) Sequence analysis and variability study of lettuce necrotic yellows virus. PhD thesis, The University of Queensland, Brisbane, Australia

  • XY Chen ZL Luo RX Fang (1998) ArticleTitleStructure analysis of the rice yellow stunt rhabdovirus gene 3 Chin Sci Bull 43 745–748

    Google Scholar 

  • TJ Choi S Kuwata EV Koonin LA Heaton AO Jackson (1992) ArticleTitleStructure of the L (polymerase) protein gene of sonchus yellow net virus Virology 189 31–39 Occurrence Handle1604816 Occurrence Handle10.1016/0042-6822(92)90678-I Occurrence Handle1:CAS:528:DyaK3sXisVyksLY%3D

    Article  PubMed  CAS  Google Scholar 

  • TJ Choi JD Wagner AO Jackson (1994) ArticleTitleSequence analysis of the trailer region of sonchus yellow net virus genomic RNA Virology 202 33–40 Occurrence Handle8009845 Occurrence Handle10.1006/viro.1994.1319 Occurrence Handle1:CAS:528:DyaK2cXltVeksLY%3D

    Article  PubMed  CAS  Google Scholar 

  • B Delecolle (1978) ArticleTitleEssais de rationalisation des méthodes de préparation d’échantillons végétaux pour la microscopie électronique: problème des précipités parasites Cell Mol Biol 23 431–436 Occurrence Handle1:STN:280:By6C1Mbhslw%3D

    CAS  Google Scholar 

  • RG Dietzgen B Callaghan T Wetzel JL Dale (2006) ArticleTitleCompletion of the genome sequence of lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus Virus Res 118 16–22 Occurrence Handle16313992 Occurrence Handle10.1016/j.virusres.2005.10.024 Occurrence Handle1:CAS:528:DC%2BD28XjvVWltbs%3D

    Article  PubMed  CAS  Google Scholar 

  • RG Dietzgen B Callaghan PR Campbell (2007) ArticleTitleBiology and genomics of lettuce necrotic yellows virus Plant Viruses 1 IssueID1 85–92

    Google Scholar 

  • RX Fang Q Wang BY Xu Z Pang HT Zhu KQ Mang DM Gao WS Qin NH Chua (1994) ArticleTitleStructure of the nucleocapsid protein gene of rice yellow stunt rhabdovirus Virology 204 367–375 Occurrence Handle8091667 Occurrence Handle10.1006/viro.1994.1541 Occurrence Handle1:CAS:528:DyaK2cXmvFCisb8%3D

    Article  PubMed  CAS  Google Scholar 

  • S Finke JH Cox KK Conzelmann (2000) ArticleTitleDifferential transcription attenuation of rabies virus genes by intergenic regions: generation of recombinant viruses over expressing the polymerase gene J Virol 74 7261–7269 Occurrence Handle10906180 Occurrence Handle10.1128/JVI.74.16.7261-7269.2000 Occurrence Handle1:CAS:528:DC%2BD3cXlt1Gku7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • Francki RIB, Randles JW, Dietzgen RG (1989) Lettuce necrotic yellows virus. In: AAB descriptions of plant viruses No 343

  • KB Goldberg B Modrell BI Hillmann LA Heaton T Choi AO Jackson (1991) ArticleTitleStructure of the glycoprotein gene of sonchus yellow net virus, a plant rhabdovirus Virology 185 32–38 Occurrence Handle1926779 Occurrence Handle10.1016/0042-6822(91)90750-6 Occurrence Handle1:CAS:528:DyaK3sXhs1Kk

    Article  PubMed  CAS  Google Scholar 

  • MM Goodin RG Dietzgen D Schichnes S Ruzin AO Jackson (2002) ArticleTitlepGD vectors: versatile tools for the expression of green and red fluorescent protein fusions in agroinfiltrated plant leaves Plant J 31 375–383 Occurrence Handle12164816 Occurrence Handle10.1046/j.1365-313X.2002.01360.x Occurrence Handle1:CAS:528:DC%2BD38XntFelsb0%3D

    Article  PubMed  CAS  Google Scholar 

  • LA Heaton D Zuidema AO Jackson (1987) ArticleTitleStructure of the M2 protein gene of sonchus yellow net virus Virology 161 234–241 Occurrence Handle3672930 Occurrence Handle10.1016/0042-6822(87)90190-5

    Article  PubMed  Google Scholar 

  • LA Heaton BI Hillman BG Hunter D Zuidema AO Jackson (1989) ArticleTitlePhysical map of the genome of sonchus yellow net virus, a plant rhabdovirus with six genes and conserved gene junction sequences Proc Natl Acad Sci USA 86 8665–8668 Occurrence Handle2813418 Occurrence Handle10.1073/pnas.86.22.8665 Occurrence Handle1:CAS:528:DyaK3cXjtFaltA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • BI Hillman LA Heaton BG Hunter B Modrell AO Jackson (1990) ArticleTitleStructure of the gene encoding the M1 protein of sonchus yellow net virus Virology 179 201–207 Occurrence Handle2219720 Occurrence Handle10.1016/0042-6822(90)90289-4 Occurrence Handle1:CAS:528:DyaK3MXhsFOrtLg%3D

    Article  PubMed  CAS  Google Scholar 

  • Y Huang H Zhao Z Luo X Chen RX Fang (2003) ArticleTitleNovel structure of the genome of rice yellow stunt virus: identification of the gene 6-encoded virion protein J Gen Virol 84 2259–2264 Occurrence Handle12867659 Occurrence Handle10.1099/vir.0.19195-0 Occurrence Handle1:CAS:528:DC%2BD3sXlvF2gt74%3D

    Article  PubMed  CAS  Google Scholar 

  • YW Huang YF Geng XB Ying XY Chen RX Fang (2005) ArticleTitleIdentification of a movement protein of rice yellow stunt rhabdovirus J Virol 79 2108–2114 Occurrence Handle15681413 Occurrence Handle10.1128/JVI.79.4.2108-2114.2005 Occurrence Handle1:CAS:528:DC%2BD2MXitlejtL4%3D

    Article  PubMed  CAS  Google Scholar 

  • AO Jackson M Goodin I Moreno J Johnson DM Lawrence (1999) Plant rhabdoviruses RG Webster A Granoff (Eds) Encyclopedia of virology Academic Press New York 1531–1541

    Google Scholar 

  • AO Jackson RG Dietzgen MM Goodin JN Bragg M Deng (2005) ArticleTitleBiology of plant rhabdoviruses Annu Rev Phytopathol 43 623–660 Occurrence Handle16078897 Occurrence Handle10.1146/annurev.phyto.43.011205.141136 Occurrence Handle1:CAS:528:DC%2BD2MXhtVOksrjK

    Article  PubMed  CAS  Google Scholar 

  • E Kretzschmar R Peluso MJ Schnell MA Whitt JK Rose (1996) ArticleTitleNormal replication of vesicular stomatitis virus without C proteins Virology 216 309–316 Occurrence Handle8607260 Occurrence Handle10.1006/viro.1996.0066 Occurrence Handle1:CAS:528:DyaK28XhtV2rt7w%3D

    Article  PubMed  CAS  Google Scholar 

  • YY Kusov G Shatirishvili G Dzagurov V Gauss-Muller (2001) ArticleTitleA new G-tailing method for the determination of the poly(A) tail length applied to hepatitis A virus RNA Nucleic Acids Res 29 IssueID12 E57 Occurrence Handle11410680 Occurrence Handle10.1093/nar/29.12.e57 Occurrence Handle1:STN:280:DC%2BD3Mzkslyhsw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • J Kyhse-Andersen (1984) ArticleTitleElectroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose J Biochem Biophys Methods 10 203–209 Occurrence Handle6530509 Occurrence Handle10.1016/0165-022X(84)90040-X Occurrence Handle1:CAS:528:DyaL2MXhs1eisrc%3D

    Article  PubMed  CAS  Google Scholar 

  • UK Laemmli (1970) ArticleTitleCleavage of structural proteins during assembly of the head of the bacteriophage T4 Nature 227 680–685 Occurrence Handle5432063 Occurrence Handle10.1038/227680a0 Occurrence Handle1:CAS:528:DC%2BD3MXlsFags7s%3D

    Article  PubMed  CAS  Google Scholar 

  • Z Luo X Chen D Gao R Fang (1998) ArticleTitleThe gene 4 of rice yellow stunt rhabdovirus encodes the matrix protein Virus Genes 16 277–280 Occurrence Handle9654681 Occurrence Handle10.1023/A:1008078605399 Occurrence Handle1:CAS:528:DyaK1cXktlKgtb4%3D

    Article  PubMed  CAS  Google Scholar 

  • ZL Luo RX Fang (1998) ArticleTitleStructure analysis of the rice yellow stunt rhabdovirus glycoprotein gene and its mRNA Arch Virol 143 2453–2459 Occurrence Handle9930201 Occurrence Handle10.1007/s007050050475 Occurrence Handle1:CAS:528:DyaK1MXhslaiu7s%3D

    Article  PubMed  CAS  Google Scholar 

  • O Poch BM Blumberg L Bougueleret N Tordo (1990) ArticleTitleSequence comparison of five polymerase (L proteins) of unsegmented negative-stranded RNA viruses: theoretical assignment of functional domains J Gen Virol 71 1153–1162 Occurrence Handle2161049 Occurrence Handle1:CAS:528:DyaK3cXksFCqurw%3D Occurrence Handle10.1099/0022-1317-71-5-1153

    Article  PubMed  CAS  Google Scholar 

  • KI Posthuma AN Adams Y Hong MJ Kirby (2002) ArticleTitleDetection of strawberry crinckle virus in plants and aphids by RT/PCR using conserved L gene sequences Plant Pathol 51 266–274 Occurrence Handle10.1046/j.1365-3059.2002.00725.x Occurrence Handle1:CAS:528:DC%2BD38XlsVGqsbY%3D

    Article  CAS  Google Scholar 

  • MG Redinbaugh SA Hogenhout (2005) ArticleTitlePlant rhabdoviruses Curr Top Microbiol Immunol 292 143–163 Occurrence Handle15981471 Occurrence Handle1:CAS:528:DC%2BD2MXnslyiu70%3D

    PubMed  CAS  Google Scholar 

  • SE Reed CW Tsai KJ Willie MG Redinbaugh SA Hogenhout (2005) ArticleTitleShotgun sequencing of the negative-sense RNA genome of the rhabdovirus maize mosaic virus J Virol Methods 129 91–96 Occurrence Handle16005085 Occurrence Handle10.1016/j.jviromet.2005.05.013 Occurrence Handle1:CAS:528:DC%2BD2MXpslCgsbY%3D

    Article  PubMed  CAS  Google Scholar 

  • P Revill X Trinh JL Dale R Harding (2005) ArticleTitleTaro vein chlorosis virus: characterization and variability of a new nucleorhabdovirus J Gen Virol 86 491–499 Occurrence Handle15659770 Occurrence Handle10.1099/vir.0.80591-0 Occurrence Handle1:CAS:528:DC%2BD2MXhtFeqsrc%3D

    Article  PubMed  CAS  Google Scholar 

  • KB Scholthof BI Hillman B Modrell LA Heaton AO Jackson (1994) ArticleTitleCharacterization and detection of sc4: a sixth gene encoded by sonchus yellow net virus Virology 204 279–288 Occurrence Handle8091658 Occurrence Handle10.1006/viro.1994.1532 Occurrence Handle1:CAS:528:DyaK2cXmsleitLw%3D

    Article  PubMed  CAS  Google Scholar 

  • CF Spiropoulou ST Nichol (1993) ArticleTitleA small highly basic protein is encoded in overlapping frame within the P gene of vesicular stomatitis virus J Virol 67 3103–3110 Occurrence Handle8388490 Occurrence Handle1:CAS:528:DyaK3sXltlaltLw%3D

    PubMed  CAS  Google Scholar 

  • F Tanno A Nakatsu S Toriyama M Kojima (2000) ArticleTitleComplete nucleotide sequence of northern cereal mosaic virus and its genome organization Arch Virol 145 1373–1384 Occurrence Handle10963343 Occurrence Handle10.1007/s007050070096 Occurrence Handle1:CAS:528:DC%2BD3cXmtVertbY%3D

    Article  PubMed  CAS  Google Scholar 

  • N Tordo A Benmansour C Calisher RG Dietzgen RX Fang AO Jackson G Kurath S Nadin-Davies RB Tesh PJ Walker (2005) Family Rhabdoviridae virus taxonomy, classification and nomenclature of viruses Academic Press San Diego

    Google Scholar 

  • CW Tsai MG Redinbaugh KJ Willie S Reed M Goodin SA Hogenhout (2005) ArticleTitleComplete genome sequence and in planta subcellular localization of maize fine streak virus proteins J Virol 79 5304–5314 Occurrence Handle15827145 Occurrence Handle10.1128/JVI.79.9.5304-5314.2005 Occurrence Handle1:CAS:528:DC%2BD2MXjs12qtL4%3D

    Article  PubMed  CAS  Google Scholar 

  • Q Wang XY Chen ZL Luo RX Fang (1999) ArticleTitleSequence analysis of leader and trailer regions of rice yellow stunt rhabdovirus and characterisation of their in vitro transcripts Sci China Ser C Life Sci 42 50–56 Occurrence Handle1:CAS:528:DyaK1MXhs1Clt7g%3D

    CAS  Google Scholar 

  • M Wassenegger S Heimes HL Sanger (1994) ArticleTitleAn infectious viroid RNA replicon evolved from an in vitro-generated non-infectious viroid deletion mutant via a complementary deletion in vivo EMBO J 13 6172–6177 Occurrence Handle7813454 Occurrence Handle1:CAS:528:DyaK2MXivFKgu74%3D

    PubMed  CAS  Google Scholar 

  • T Wetzel RG Dietzgen JL Dale (1994) ArticleTitleGenomic organization of lettuce necrotic yellows rhabdovirus Virology 200 401–412 Occurrence Handle8178430 Occurrence Handle10.1006/viro.1994.1204 Occurrence Handle1:CAS:528:DyaK2cXlslWksbs%3D

    Article  PubMed  CAS  Google Scholar 

  • T Wetzel RG Dietzgen AD Geering JL Dale (1994) ArticleTitleAnalysis of the nucleocapsid gene of lettuce necrotic yellows rhabdovirus Virology 202 1054–1057 Occurrence Handle8030210 Occurrence Handle10.1006/viro.1994.1438 Occurrence Handle1:CAS:528:DyaK2cXmsVOmu7w%3D

    Article  PubMed  CAS  Google Scholar 

  • HT Zhu XY Chen ZL Luo RX Fang DM Gao (1997) ArticleTitleNucleotide sequence of the rice yellow stunt rhabdovirus gene 2 Chin J Virol 13 369–375 Occurrence Handle1:CAS:528:DyaK1cXit1yktL0%3D

    CAS  Google Scholar 

  • D Zuidema LA Heaton R Hanau AO Jackson (1986) ArticleTitleDetection and sequence of plus-strand leader RNA of sonchus yellow net virus, a plant rhabdovirus Proc Natl Acad Sci USA 83 5019–5023 Occurrence Handle16593726 Occurrence Handle10.1073/pnas.83.14.5019 Occurrence Handle1:CAS:528:DyaL28Xlt1ynt7c%3D

    Article  PubMed  CAS  Google Scholar 

  • D Zuidema LA Heaton AO Jackson (1987) ArticleTitleStructure of the nucleocapsid protein gene of sonchus yellow net virus Virology 159 373–380 Occurrence Handle10.1016/0042-6822(87)90476-4 Occurrence Handle1:CAS:528:DyaL2sXmtlShtr4%3D Occurrence Handle18644572

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. RLP Agroscience, AlPlanta – Institute for Plant Research, Neustadt an der Weinstrasse, Germany

    F. Heim, A. Bassler, G. Krczal & T. Wetzel

  2. I.N.R.A., Station de Pathologie Végétale, Domaine St Maurice, Montfavet, France

    H. Lot & B. Delecolle

Authors
  1. F. Heim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Lot
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Delecolle
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Bassler
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Krczal
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. T. Wetzel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. Wetzel.

Additional information

Correspondence: Thierry Wetzel, RLP Agroscience, AlPlanta – Institute for Plant Research, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heim, F., Lot, H., Delecolle, B. et al. Complete nucleotide sequence of a putative new cytorhabdovirus infecting lettuce. Arch Virol 153, 81–92 (2008). https://doi.org/10.1007/s00705-007-1071-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-007-1071-5

Keywords

Search

Navigation