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Genome sequence of the novel freshwater Microcystis cyanophage Mwe-Yong1112-1

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Abstract

The freshwater cyanophage Mwe-Yong1112-1 was isolated using Microcystis wesenbergii as a host and found to have an icosahedral head, about 45 nm in diameter, and a flexible tail, approximately 133 nm in length and 4.5 nm in width. The complete genome of the cyanophage is 39,679 bp in length with a G+C content of 66.6%. Mwe-Yong1112-1 shared the highest pairwise average nucleotide identity (ANI) value of 67.7% (below the ≥95% boundary to define a species) and the highest nucleotide sequence similarity of 17.48% (below the >70% boundary to define a genus) with the most closely related phage. In a proteomic tree, Mwe-Yong1112-1 and three unclassified phages formed a monophyletic clade between the families Saparoviridae and Pyrstoviridae, but Mwe-Yong1112-1 occupied a separate branch from the other three phages, suggesting that it represents a new evolutionary lineage. This study enriches the available information about freshwater cyanophages.

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Acknowledgements

We are grateful for the high-quality technical support provided by Pingping Zhan of the Electron Microscopy Laboratory of Ningbo University.

Funding

This study was funded by the National Key Research and Development Program (2018YFA0903000) and the Open Fund of Key Laboratory of Biogenetic Resources of the State Oceanic Administration (HY201602) and sponsored by the K. C. Wong Magna Fund of Ningbo University.

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Author notes

  1. Ruqian Cai and Dengfeng Li contributed equally to this work.

Authors and Affiliations

  1. School of Marine Sciences, Ningbo University, Ningbo, 315211, China

    Ruqian Cai, Dengfeng Li, Wei Lin, Weinan Qin, Lingting Pan, Fei Wang, Minhua Qian, Wencai Liu & Qin Zhou

  2. College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315832, China

    Ruqian Cai & Chengxu Zhou

  3. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

    Wei Lin & Yigang Tong

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Contributions

DL, RC, and YT designed the research. RC, DL, WL, WQ, LP, FW, MQ, WL, QZ, CZ, and YT performed the research. RC and DL analyzed data and wrote the paper. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Dengfeng Li.

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Supplementary Information

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Supplementary Fig. S1

(a) Transparent circular plaques formed by cyanophage Mwe-Yong1112-1 on an M. wesenbergii lawn. (b) Macrograph of normal FACHB-1112 cultures (1112 C) and FACHB-1112 co-inoculated with cyanophage Mwe-Yong1112-1 (1112 T). Supplementary file1 (TIF 23201 KB)

Supplementary file2 (DOCX 18 KB)

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Cai, R., Li, D., Lin, W. et al. Genome sequence of the novel freshwater Microcystis cyanophage Mwe-Yong1112-1. Arch Virol 167, 2371–2376 (2022). https://doi.org/10.1007/s00705-022-05542-3

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