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Complete genome sequence of a Moroccan isolate of cereal chlorotic mottle virus

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Abstract

Cereal chlorotic mottle virus (CCMoV) is a cicadellid-transmitted plant rhabdovirus associated with chlorotic and necrotic streaks on several gramineous hosts and weeds. The virus was initially described in 1979 in Australia, but its genome has never been sequenced. In this study, the complete genome sequence of a Moroccan isolate of CCMoV was generated by high-throughput sequencing from infected oat leaves (Avena sativa). The genome is 13,800 nt long, containing seven open reading frames (ORFs) arranged in the canonical organization of rhabdoviruses: 3’-nucleocapsid (N), phosphoprotein (P), unknown protein (p3), unknown protein (p4), matrix (M), glycoprotein (G), viral polymerase (L)-5’. Pairwise analysis showed that maize fine streak virus (MFSV, genus Gammanucleorhabdovirus) was the closest relative. The amino acid identity values between homologous proteins from CCMoV and MFSV are as follows: 59.27% (N), 36.7% (P), 24% (P3), 62% (P4), 43.70% (M), 49.15% (G), 60.93% (L). Based on its phylogenetic relationship and analogous genome architecture, CCMoV should be assigned as member of the genus Gammanucleorhabdovirus. The low sequence similarity observed between CCMoV and MFSV suggests that CCMoV is a member of a distinct virus species.

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Data availability

The genomic sequence determined in this study was submitted to the GenBank database under the accession number MW731536.

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Acknowledgements

Robert A. Alvarez-Quinto is supported by the Republic of Ecuador through the Ecuadorean Science and Technology Secretariat (SENESCYT).

Funding

This work was supported by the USDA National Institute of Food and Agriculture, Hatch project #1009997 and the University of Minnesota, Twin Cities.

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Authors and Affiliations

  1. Department of Botany and Plant Pathology, Cordley Hall, 2701 SW Campus Way, Corvallis, OR, 97331, USA

    Robert A. Alvarez-Quinto

  2. Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN, 55108, USA

    Robert A. Alvarez-Quinto, Benham E. Lockhart & Joana Serrano

  3. USDA-ARS National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA

    Samuel Grinstead

  4. USDA-ARS Horticultural Crops Disease and Pest Management Research Unit, Corvallis, OR, 97330, USA

    Dimitre Mollov

Authors
  1. Robert A. Alvarez-Quinto
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  2. Benham E. Lockhart
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  3. Joana Serrano
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  4. Samuel Grinstead
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  5. Dimitre Mollov
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Contributions

Conceptualization: Benham E. Lockhart, Dimitre Mollov. Formal analysis: Robert A. Alvarez-Quinto, Samuel Grinstead. Investigation: Robert A. Alvarez-Quinto, Benham E. Lockhart, Joana Serrano. Methodology: Benham E. Lockhart, Dimitre Mollov. Supervision: Benham E. Lockhart. Writing—original draft: Robert A. Alvarez-Quinto. Writing—review & editing: Robert A. Alvarez-Quinto, Benham E. Lockhart, Joana Serrano, Samuel Grinstead, Dimitre Mollov. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Robert A. Alvarez-Quinto.

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The authors have no relevant financial or non-financial interests to disclose.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Ralf Georg Dietzgen.

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Supplementary Information

Below is the link to the electronic supplementary material.

705_2022_5527_MOESM1_ESM.jpg

Supplementary file1 Supplementary Fig. S1 Sequence alignment of the gene junctions detected in cereal chlorotic mottle virus (CCMoV-M) and maize fine streak virus (MFSV). All sequence differences are highlighted in black (JPG 69 KB)

Supplementary file2 (FASTA 14 KB)

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Alvarez-Quinto, R.A., Lockhart, B.E., Serrano, J. et al. Complete genome sequence of a Moroccan isolate of cereal chlorotic mottle virus. Arch Virol 167, 2347–2350 (2022). https://doi.org/10.1007/s00705-022-05527-2

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  • DOI: https://doi.org/10.1007/s00705-022-05527-2

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