Abstract
A rhabdovirus infecting maize and wheat crops in Argentina was molecularly characterized. Through next-generation sequencing (NGS) of symptomatic leaf samples, the complete genome was obtained of two isolates of maize yellow striate virus (MYSV), a putative new rhabdovirus, differing by only 0.4% at the nucleotide level. The MYSV genome consists of 12,654 nucleotides for maize and wheat virus isolates, and shares 71% nucleotide sequence identity with the complete genome of barley yellow striate mosaic virus (BYSMV, NC028244). Ten open reading frames (ORFs) were predicted in the MYSV genome from the antigenomic strand and were compared with their BYSMV counterparts. The highest amino acid sequence identity of the MYSV and BYSMV proteins was 80% between the L proteins, and the lowest was 37% between the proteins 4. Phylogenetic analysis suggested that the MYSV isolates are new members of the genus Cytorhabdovirus, family Rhabdoviridae. Yellow striate, affecting maize and wheat crops in Argentina, is an emergent disease that presents a potential economic risk for these widely distributed crops.
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References
USDA (2017) United States Department of Agriculture. In: Prod. Supply Distrib. Online. https://apps.fas.usda.gov/psdonline/app/index.html#/app/downloads
Maurino MF, Laguna G, Giolitti F et al (2012) First ocurrence of a rhabdovirus infecting maize in Argentina. Plant Dis 96:1383
Dumón AD, Mattio MF, Argüello-Caro EB et al (2015) Occurrence of a closely-related isolate to Maize yellow striate virus in wheat plants. Agriscientia 32:107–112
Tritt A, Eisen JA, Facciotti MT, Darling AE (2012) An integrated Pipeline for de novo assembly of microbial genomes. PLoS One. doi:10.1371/journal.pone.0042304
Kearse M, Moir R, Wilson A et al (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649. doi:10.1093/bioinformatics/bts199
Milne I, Stephen G, Bayer M et al (2013) Using Tablet for visual exploration of second-generation sequencing data. Brief Bioinform 14:193–202. doi:10.1093/bib/bbs012
Lander ES, Waterman MS (1988) Genomic mapping by fingerprinting random clones: a mathematical analysis. Genomics 2:231–239
Frohman MA, Dush MK, Martin GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85:8998–9002. doi:10.1073/pnas.85.23.8998
Yan T, Zhu J-R, Di D et al (2015) Characterization of the complete genome of Barley yellow striate mosaic virus reveals a nested gene encoding a small hydrophobic protein. Virology 478:112–122
Tanno F, Nakatsu A, Toriyama SS, Koyima M (2000) Complete nucleotide sequence of Northern cereal mosaic virus and its genome organization. Arch Virol 145:1373–1384
Callaghan B, Dietzgen RG (2005) Nucleocapsid gene variability reveals two subgroups of Lettuce necrotic yellows virus. Arch Virol 150:1661–1667. doi:10.1007/s00705-005-0528-7
Revill P, Trinh X, Dale J, Harding R (2005) Taro vein chlorosis virus: characterization and variability of a new Nucleorhabdovirus. J Gen Virol 86:491–499. doi:10.1099/vir.0.80591-0
Klerks MM, Lindner JL, Vaskova D et al (2004) Detection and tentative grouping of Strawberry crinkle virus isolates. Eur J Plant Pathol 110:45–52
Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704. doi:10.1080/10635150390235520
Dietzgen RG, Kuhn JH, Clawson AN et al (2014) Dichorhavirus: a proposed new genus for Brevipalpus mite-transmitted, nuclear, bacilliform, bipartite, negative-strand RNA plant viruses. Arch Virol 159:607–619. doi:10.1007/s00705-013-1834-0
Kondo H, Maeda T, Shirako Y, Tamada T (2006) Orchid fleck virus is a rhabdovirus with an unusual bipartite genome. J Gen Virol 87:2413–2421. doi:10.1099/vir.0.81811-0
Ramalho TO, Figueira AR, Sotero AJ et al (2014) Characterization of Coffee ringspot virus-Lavras: a model for an emerging threat to coffee production and quality. Virology 464–465:385–396. doi:10.1016/j.virol.2014.07.031
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This work was funded by INTA Research Projects (PNPV-1135022 INTA) and Fundación ArgenINTA.
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Author María Fernanda Maurino has received research grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Author Analía Delina Dumón has received research grants from Fondo para la Investigación Científica y Tecnológica (FONCyT). Authors Vanina Alemandri, Gabriela Llauger, Luis A. de Haro, M. Fernanda Mattio, Mariana del Vas, Irma Graciela Laguna and María de la Paz Giménez Pecci declare that they have no conflict of interest.
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Maurino, F., Dumón, A.D., Llauger, G. et al. Complete genome sequence of maize yellow striate virus, a new cytorhabdovirus infecting maize and wheat crops in Argentina. Arch Virol 163, 291–295 (2018). https://doi.org/10.1007/s00705-017-3579-7
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DOI: https://doi.org/10.1007/s00705-017-3579-7