Skip to main content
SpringerLink
Log in

Molecular characterization of a novel virga-like virus associated with wheat

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

In this work, we report the detection of a novel single-strand RNA virus from wheat, tentatively named "Triticum aestivum-associated virga-like virus 1" (TaAVLV1). Further characterization revealed that the complete genome of TaAVLV1 is divided into two segments, RNA1 and RNA2, which are 3530 and 3466 nt in length, excluding their respective polyA tails, and each contains only one open reading frame (ORF). The ORF of RNA1 encodes an RNA-dependent RNA polymerase (RdRp), while the ORF of RNA2 encodes a putative protein with methyltransferase and helicase domains. Phylogenetic analysis showed that the RdRp of TaAVLV1 is closely related to those of members of the unclassified virga-like virus group in the family Virgaviridae. Thus, we have identified TaAVLV1 as a putative novel virga-like virus belonging to the family Virgaviridae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Shewry PR, Hey SJ (2015) The contribution of wheat to human diet and health. Food Energy Secur 4:178–202. https://doi.org/10.1002/fes3.64

    Article  PubMed  PubMed Central  Google Scholar 

  2. Kühne T (2009) Soil-borne viruses affecting cereals: known for long but still a threat. Virus Res 141:174–183. https://doi.org/10.1016/j.virusres.2008.05.019

    Article  CAS  PubMed  Google Scholar 

  3. Chen JJ (2010) Occurrence of fungally transmitted wheat mosaic viruses in China. Ann Appl Biol 123:55–61. https://doi.org/10.1111/j.1744-7348.1993.tb04072.x

    Article  Google Scholar 

  4. Liu Y, Khine MO, Zhang P, Fu Y, Wang X (2020) Incidence and distribution of insect-transmitted cereal viruses in wheat in China from 2007 to 2019. Plant Dis 104:1407–1414. https://doi.org/10.1094/PDIS-11-19-2323-RE

    Article  CAS  PubMed  Google Scholar 

  5. Han C, Li D, Xing Y, Zhu K, Tian Z, Cai Z, Yu J, Liu Y (2000) Wheat yellow mosaic virus widely occurring in wheat (Triticum aestivum) in China. Plant Dis 84:627–630. https://doi.org/10.1094/PDIS.2000.84.6.627

    Article  PubMed  Google Scholar 

  6. Zhang F, Liu S, Zhang T, Ye Z, Han X, Zhong K, Yang J, Chen J, Liu P (2021) Construction and biological characterization of an infectious full-length cDNA clone of a Chinese isolate of Wheat yellow mosaic virus. Virology 556:101–109. https://doi.org/10.1016/j.virol.2021.01.018

    Article  CAS  PubMed  Google Scholar 

  7. Liu-ming GUO, Jing HE, Jing LI, Jian-ping CHEN, Agriculture ZHANG H-mJIA (2019) Chinese wheat mosaic virus: a long-term threat to wheat in China.  https://doi.org/10.1016/S2095-3119(18)62047-7

  8. Adams MJ, Antoniw JF, Kreuze J (2009) Virgaviridae: a new family of rod-shaped plant viruses. Arch Virol 154:1967–1972. https://doi.org/10.1007/s00705-009-0506-6

    Article  CAS  PubMed  Google Scholar 

  9. Adams MJ, Adkins S, Bragard C, Gilmer D, Li D, MacFarlane SA, Wong SM, Melcher U, Ratti C, Ryu KH, Ictv Report C (2017) ICTV virus taxonomy profile: Virgaviridae. J Gen Virol 98:1999–2000. https://doi.org/10.1099/jgv.0.000884

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Thekke-Veetil T, Lagos-Kutz D, McCoppin NK, Hartman GL, Ju HK, Lim HS, Domier LL (2020) Soybean Thrips (Thysanoptera: Thripidae) Harbor Highly Diverse Populations of Arthropod, Fungal and Plant Viruses. https://doi.org/10.3390/v12121376. Viruses 12

  11. Quintanilha-Peixoto G, Fonseca PLC, Raya FT, Marone MP, Bortolini DE, Mieczkowski P, Olmo RP, Carazzolle MF, Voigt CA, Soares ACF, Pereira GAG, Góes-Neto A, Aguiar E (2021) The Sisal Virome: uncovering the viral diversity of Agave varieties reveals new and organ-specific viruses. Microorganisms. https://doi.org/10.3390/microorganisms9081704

    Article  PubMed  PubMed Central  Google Scholar 

  12. Chiapello M, Rodríguez-Romero J, Ayllón MA, Turina M (2020) Analysis of the virome associated to grapevine downy mildew lesions reveals new mycovirus lineages. Virus Evol 6:veaa058. https://doi.org/10.1093/ve/veaa058

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Hadidi A, Flores R, Candresse T, Barba M (2016) Next-generation sequencing and Genome editing in plant virology. Front Microbiol 7:1325. https://doi.org/10.3389/fmicb.2016.01325

    Article  PubMed  PubMed Central  Google Scholar 

  14. Kondo H, Yoshida N, Fujita M, Maruyama K, Hyodo K, Hisano H, Tamada T, Andika IB, Suzuki N (2021) Identification of a Novel Quinvirus in the Family Betaflexiviridae That Infects Winter Wheat. Front Microbiol 12:715545. https://doi.org/10.3389/fmicb.2021.715545

    Article  PubMed  PubMed Central  Google Scholar 

  15. Fu S, Zhang T, He M, Sun B, Zhou X, Wu J (2021) Molecular characterization of a novel wheat-infecting virus of the family Betaflexiviridae. Arch Virol 166:2875–2879. https://doi.org/10.1007/s00705-021-05175-y

    Article  CAS  PubMed  Google Scholar 

  16. Redila CD, Prakash V, Nouri S (2021) Metagenomics Analysis of the Wheat Virome Identifies Novel Plant and Fungal-Associated Viral Sequences. Viruses 13:2457. https://doi.org/10.3390/v13122457

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Andika IB, Wei S, Cao C (2017) Phytopathogenic fungus hosts a plant virus: a naturally occurring cross-kingdom viral infection. Proc Natl Acad Sci 114:12267–12272. https://doi.org/10.1073/pnas.1714916114

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Bian R, Andika IB, Pang T (2020) Facilitative and synergistic interactions between fungal and plant viruses. Proc Natl Acad Sci 117:3779–3788. https://doi.org/10.1073/pnas.1915996117

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgments

The authors thank Professor K.Q. Tang (USA) for manuscript correction.

Funding

This work was supported by the National Key R&D Plan in China (2018YFD0200408, 2018YFD0200507, 2017YFD-0201701), the China Agriculture Research System from the Ministry of Agriculture of the P.R. China (CARS-03), the National Key Project for Research on Transgenic Biology (2016ZX08002-001), the International Science and Technology Cooperation Program of China (2012DFA30900), and the K.C. Wong Magna Fund of Ningbo University.

Author information

Author notes

  1. Hua Li and Jun Guo contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China

    Hua Li, Jun Guo, Zhuangxin Ye, Jianping Chen & Jian Yang

  2. National Agro-Technical Extension and Service Center, Beijing, 100125, China

    ZhongHua Zhao

Authors
  1. Hua Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jun Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhuangxin Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. ZhongHua Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jianping Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jian Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Jianping Chen or Jian Yang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: Robert H. A. Coutts.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 15 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, H., Guo, J., Ye, Z. et al. Molecular characterization of a novel virga-like virus associated with wheat. Arch Virol 167, 1909–1913 (2022). https://doi.org/10.1007/s00705-022-05473-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-022-05473-z

Search

Navigation