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Metabolic characterization of hemolymph in Bombyx mori varieties after Bombyx mori nucleopolyhedrovirus infection by GC-MS-based metabolite profiling

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Abstract

The "Huakang 2" silkworm variety, bred by the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, is highly resistant to Bombyx mori nucleopolyhedrovirus (BmNPV) and effectively solves the issue of frequent Bombyx mori nuclear polyhedrosis in sericultural production. The molecular mechanism of its resistance to BmNPV, however, is still unknown. The purpose of the present study was therefore to identify these anti-BmNPV mechanisms by using metabolomics in combination with transcriptomics after subcutaneous injection of budded virus (BV) with high concentrations of BmNPV from specimens of the Baiyu N variety (which is highly resistant to BmNPV) and the Baiyu variety (which is sensitive to BmNPV). A total of 375 differential metabolites were identified, which mainly included sugars, acids, amines, alcohols, glycosides, and other small molecules. KEGG enrichment analysis and functional clustering of differential metabolites identified possible metabolic pathways, including tyrosine metabolism, oxidative phosphorylation, and alanine, aspartate, and glutamate metabolism. The differentially expressed genes (DEGs) identified by transcriptome analysis were annotated in KEGG. Association analysis showed that the metabolic pathways of different silkworm varieties are affected differently by BmNPV infection, triggering a series of complex physiological and biochemical changes in the organism. In particular, oxidative phosphorylation might be an essential pathway involved in regulation of disease resistance.

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Acknowledgments

We would like to thank TopEdit (www.topeditsci.com) for English language editing of this manuscript.

Funding

This work was supported by the Key R&D Plan of Jiangsu Province (Modern Agriculture, BE2020418), the National Natural Science Foundation of China (no. 31802316), and the China Agriculture Research System of MOF and MARA.

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Authors and Affiliations

  1. The Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212003, China

    Heying Qian, Huimin Guo, Xiao Zhang, Guodong Zhao, Anying Xu & Gang Li

  2. The key Laboratory of silkworm and mulberry genetic improvement, Ministry of Agriculture, Chinese Academy of Agricultural Science, Zhenjiang, 212018, China

    Heying Qian, Mingzhu Liu, Guodong Zhao, Anying Xu & Gang Li

Authors
  1. Heying Qian
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  2. Huimin Guo
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  3. Xiao Zhang
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  4. Mingzhu Liu
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  5. Guodong Zhao
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  6. Anying Xu
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  7. Gang Li
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Contributions

Conceived and designed the experiments: HYQ. Performed the experiment: HMG, XZ, MZL, GDZ. Analyzed the data: HYQ, HMG and GL. Wrote the paper: HYQ and GL.

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Correspondence to Heying Qian or Gang Li.

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The authors declare no conflict of interest.

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Handling Editor: Simona Abba'.

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Qian, H., Guo, H., Zhang, X. et al. Metabolic characterization of hemolymph in Bombyx mori varieties after Bombyx mori nucleopolyhedrovirus infection by GC-MS-based metabolite profiling. Arch Virol 167, 1637–1648 (2022). https://doi.org/10.1007/s00705-022-05463-1

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  • DOI: https://doi.org/10.1007/s00705-022-05463-1

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