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Protein divergence in Helicoverpa armigera hemolymph induced by the dietary triflumuron alone and in binary combination with β-sitosterol

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Abstract

The current study investigated proteomic divergence in the hemolymph of early fourth instar larvae of Helicoverpa armigera fed with triflumuron (TFM) and a synergistic growth inhibitory combination of TFM and β-sitosterol (BS) in a 1:1 ratio. LC-MS analysis identified 65 proteins differentially downregulated in larvae fed with TFM and 75 in larvae fed with TFM + BS, essential for maintaining homeostasis and performing physiological and metabolic activities in H. armigera, when compared to untreated controls. In the TFM-fed larvae, deletions comprised 7 DNA processing, 11 RNA processing, 7 protein processing, 6 metabolic, 5 transport, 11 structural, 8 signal transduction, and 10 stress response proteins. TFM + BS-fed larvae showed relative deletions of 8 DNA processing, 14 RNA processing, 6 protein processing, 7 metabolic, 7 transport, 13 structural proteins, 7 signal transduction, and 12 stress response proteins. Both diets resulted in differential expression of 10 and 6 proteins, respectively, with TFM-fed larvae expressing proteins related to defense and detoxification, while TFM + BS-fed larvae expressing primarily upregulated proteins involved in sensory stimulus perception, particularly olfaction, along with one stress protein. Thus, analysis of the overall protein expression of the organism could provide an insight into important proteins suppressed and activated which can be future targets protein to control the pest population.

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Acknowledgements

The authors thank to the Principal, Acharya Narendra Dev College for providing the laboratory and culture facilities to conduct the experimental work.

Funding

The authors are grateful to the Council of Scientific and Industrial Research, New Delhi [Grant No. 13(9163-A)/2021-Pool] for providing the research fellowship to carry out the investigations.

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Authors and Affiliations

  1. Department of Zoology, Acharya Narendra Dev College (University of Delhi), Kalkaji, 110019, New Delhi, India

    Monika Mishra, Aarti Sharma, Vinay Singh Dagar & Sarita Kumar

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  1. Monika Mishra
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  2. Aarti Sharma
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  3. Vinay Singh Dagar
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Contributions

All the authors contributed to the study conception and design. Material preparation, experimentation and data collection were performed by Monika Mishra, Aarti Sharma and Vinay Singh Dagar. The results were critically analysed by Monika Mishra and Sarita Kumar. The first draft of the manuscript was written by Monika Mishra and all authors contributed to the editing of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Monika Mishra.

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Mishra, M., Sharma, A., Dagar, V.S. et al. Protein divergence in Helicoverpa armigera hemolymph induced by the dietary triflumuron alone and in binary combination with β-sitosterol. Int J Trop Insect Sci (2024). https://doi.org/10.1007/s42690-024-01234-4

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