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Construction of an infectious full-length and eGFP-tagged cDNA clone of a chilli ringspot virus isolate from Yunnan province, China

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Abstract

Chilli ringspot virus (ChiRSV; genus Potyvirus) was one of several viruses previously detected in pepper samples with severe yellowing and curling symptoms growing in Wenshan, Yunan province, China. We now report the full-length sequence of ChiRSV-YN/Wenshan (MZ269480), which has 88.5-98.9% nucleotide sequence identity to other published ChiRSV isolates. A full-length cDNA infectious clone was constructed. This cDNA and an eGFP-tagged clone were infectious, leading to systemic symptoms in both Nicotiana benthamiana and Capsicum spp. Recombinant clones containing the P1 protein coding region of other ChiRSV isolates differed in their pathogenicity. Single infection by ChiRSV caused mild mosaic or leaf crinkling in Capsicum frutescens L. and Capsicum annuum L.

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Data availability statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgments

This work was supported by the Chinese Agriculture Research System of MOF and MARA (CARS-24-C-04). This work was also financially supported by the National Key R&D Program of China (2019YFD1001800) and the K. C. Wong Education Foundation. We thank Professor Mike Adams for manuscript correction.

Funding

This work was supported by the Chinese Agriculture Research System of MOF and MARA (CARS-24-C-04). This work was also financially supported by the National Key R&D Program of China (2019YFD1001800) and the K. C. Wong Education Foundation. We thank Professor Mike Adams for manuscript correction.

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Author notes

  1. Mengying Hua and Shanshan Jiang have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Institute of Plant Virology, Ningbo University, Ningbo, 315211, Zhejiang, China

    Mengying Hua, Yuwen Lu, Hongying Zheng, Jianping Chen, Fei Yan & Jiejun Peng

  2. Shandong Provincial Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong, China

    Shanshan Jiang & Liyan Wang

  3. Wenshan Academy of Agricultural Sciences, Wenshan, 663000, Yunnan, China

    Enping Yuan & Shaoxiang Wang

  4. College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Qionglian Wan & Hairu Chen

Authors
  1. Mengying Hua
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  2. Shanshan Jiang
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  3. Enping Yuan
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  4. Qionglian Wan
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  5. Liyan Wang
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  9. Jianping Chen
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  10. Fei Yan
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  11. Shaoxiang Wang
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Contributions

MH, SJ, SW, and JP conceived and designed the experiments. EY, HZ, QW, YL, and HC collected the samples. MH and SJ performed the experiments. FY and JC analyzed the data. MH, SJ, SW, and JP wrote the paper. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Shaoxiang Wang or Jiejun Peng.

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The authors declare that they have no conflict of interest.

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Hua, M., Jiang, S., Yuan, E. et al. Construction of an infectious full-length and eGFP-tagged cDNA clone of a chilli ringspot virus isolate from Yunnan province, China. Arch Virol 167, 1583–1587 (2022). https://doi.org/10.1007/s00705-022-05457-z

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  • DOI: https://doi.org/10.1007/s00705-022-05457-z

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