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Complete genome sequence of a novel fusarivirus from the phytopathogenic fungus Corynespora cassiicola

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Abstract

Corynespora cassiicola is an important phytopathogenic fungus that severely impairs crop production. Here, we report the molecular characterization of a novel positive-sense single-stranded RNA (+ssRNA) mycovirus, Corynespora cassiicola fusarivirus 1 (CcFV1), isolated from C. cassiicola strain 20200826-3-1. Excluding the poly(A) tail, the genome of the virus is 6491 nt in length and contains three putative open reading frames (ORFs). The large ORF1 encodes a polypeptide of 1524 aa with a conserved RNA-dependent RNA polymerase (RdRp) domain and a helicase (Hel) domain. BLASTp analysis showed that CcFV1 ORF1 has the highest similarity to Setosphaeria turcica fusarivirus 1 (StFV1, 50.45% identity, E-value 0.0). ORF2 encodes a polypeptide with a conserved chromosome segregation ATPase (Smc) domain. The smaller ORF3 encodes a polypeptide with an unknown function. Phylogenetic analysis based on the ORF1- encoded polypeptide showed that CcFV1 is phylogenetically related to members of the newly proposed family "Fusariviridae". Thus, we suggest that CcFV1 might be a novel member of the family "Fusariviridae", and is the first to be discovered in C. cassiicola.

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Acknowledgments

We are extremely grateful to Dr. Jing Wang for her valuable suggestions and help with sequence analysis.

Funding

This work was supported by the China Agriculture Research System of MOF and MARA [Grant number CARS-14] and the Key Project of Science and Technology of Henan Province [Grant number 201300110600].

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Authors and Affiliations

  1. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Postgraduate T&R Base of Zhengzhou University, Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Key Laboratory of Crop Pest Control, Zhengzhou, 450002, Henan, China

    Mingming Liu, Xintao Liu, Hui Zhao, Yunxia Ni, Min Jia, Peilin Hu & Hongyan Liu

  2. School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China

    Mingming Liu, Peilin Hu, Hongyan Liu & Baoming Tian

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  1. Mingming Liu
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  2. Xintao Liu
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  3. Hui Zhao
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  4. Yunxia Ni
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  5. Min Jia
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  6. Peilin Hu
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  7. Hongyan Liu
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Correspondence to Hongyan Liu or Baoming Tian.

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Liu, M., Liu, X., Zhao, H. et al. Complete genome sequence of a novel fusarivirus from the phytopathogenic fungus Corynespora cassiicola. Arch Virol 167, 1375–1379 (2022). https://doi.org/10.1007/s00705-022-05428-4

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  • DOI: https://doi.org/10.1007/s00705-022-05428-4

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