Abstract
A novel wheat-infecting polerovirus, tentatively named “wheat yellow dwarf virus” (WYDV), was identified from winter wheat in China using transcriptome sequencing (RNA-seq) combined with RT-PCR and RACE amplification. WYDV has a single-stranded RNA genome of 5,650 nucleotides (nt) and contains seven putative open reading frames (ORFs). WYDV was found to share the highest sequence identity with cereal yellow dwarf virus RPV (CYDV-RPV, genus Polerovirus), 71.1% for the nucleotide sequence of the whole genome, and 77.3% and 70.0% for the amino acid sequences of the coat protein (CP) and RNA-dependent RNA polymerase protein (RdRp), respectively. Phylogenetic analysis based on the complete genome and CP and RdRp amino acid sequences showed that WYDV is most closely related to the cereal-infecting poleroviruses CYDV-RPV, CYDV-RPS, and barley yellow dwarf virus-GPV. These data suggest that WYDV, which is associated with a newly emerging yellow dwarf disease in wheat fields in central China, should be classified as a new member of the genus Polerovirus.
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Nucleotide sequence data reported in this study are available in the GenBank database under the accession number OK216142.
References
Gray S, Gildow FE (2003) Luteovirus-aphid interactions. Annu Rev Phytopathol 41:539–566
Domier LL (2012) Family-Luteoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, 9th report of the ICTV. Elsevier/Academic Press, London, pp 1045–1053
Vincent JR, Lister RM, Larkins BA (1991) Nucleotide sequence analysis and genomic organization of the NY-RPV isolate of barley yellow dwarf virus. J Gen Virol 72(Pt 10):2347–2355
Almasi R, Miller WA, Ziegler-Graff V (2015) Mild and severe cereal yellow dwarf viruses differ in silencing suppressor efficiency of the P0 protein. Virus Res 208:199–206
Krueger EN, Beckett RJ, Gray SM, Miller WA (2013) The complete nucleotide sequence of the genome of barley yellow dwarf virus-RMV reveals it to be a new polerovirus distantly related to other yellow dwarf viruses. Front Microbiol 4:205
Chen S, Jiang GZ, Wu JX, Liu Y, Qian YJ, Zhou XF (2016) Characterization of a novel polerovirus infecting maize in China. Viruses 8:120
Moonan F, Molina J, Mirkov TE (2000) Sugarcane yellow leaf virus: an emerging virus that has evolved by recombination between luteoviral and poleroviral ancestors. Virology 269:156–171
Zhang WW, Cheng ZM, Xu L, Wu MS, Waterhouse P, Zhou GH, Li SF (2009) The complete nucleotide sequence of the barley yellow dwarf GPV isolate from China shows that it is a new member of the genus Polerovirus. Arch Virol 154:1125–1128
Zhang PP, Liu Y, Liu WW, Cao MJ, Massart S, Wang XF (2017) Identification, characterization and full-length sequence analysis of a novel polerovirus associated with wheat leaf yellowing disease. Front Microbiol 8:1689
Zhao F, Lim S, Yoo RH, Igori D, Kim SM, Kwak do Y, Kim SL, Lee BC, Moon JS (2016) The complete genomic sequence of a tentative new polerovirus identified in barley in South Korea. Arch Virol 161:2047–2050
Liu Y, Du ZZ, Wang H, Zhang S, Cao MJ, Wang XF (2018) Identification and characterization of wheat yellow striate virus, a novel leafhopper-transmitted nucleorhabdovirus infecting wheat. Front Microbiol 9:468
Zhu T, Wang L, Rimbert H, Rodriguez JC, Deal KR, De Oliveira R, Choulet F, Keeble-Gagnere G, Tibbits J, Rogers J, Eversole K, Appels R, Gu YQ, Mascher M, Dvorak J, Luo MC (2021) Optical maps refine the bread wheat Triticum aestivum cv. Chinese Spring genome assembly. Plant J 107(1):303–314
Liu Y, Zhai H, Zhao K, Wu BL, Wang XF (2012) Two suppressors of RNA silencing encoded by cereal-infecting members of the family Luteoviridae. J Gen Virol 93:1825–1830
Smirnova E, Firth AE, Miller WA, Scheidecker D, Brault V, Reinbold C, Rakotondrafara AM, Chung BY, Ziegler-Graff V (2015) Discovery of a small non-AUG-initiated ORF in poleroviruses and luteoviruses that is required for long-distance movement. PLoS Pathog 11:e1004868
van der Wilk F, Verbeek M, Dullemans AM, van den Heuvel JF (1997) The genome-linked protein of potato leafroll virus is located downstream of the putative protease domain of the ORF1 product. Virology 234:300–303
Brown CM, Dinesh-Kumar SP, Miller WA (1996) Local and distant sequences are required for efficient readthrough of the barley yellow dwarf virus PAV coat protein gene stop codon. J Virol 70:5884–5892
Tamura K, Stecher G, Kumar S (2021) MEGA11: Molecular evolutionary genetics analysis version 11. Mol Biol Evol 38(7):3022–3027
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This work was financially supported by the National Natural Science Foundation of China (31871938 and 31861133020).
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Guo, M., Yuan, X., Wu, N. et al. Complete genome sequence of a novel wheat-infecting polerovirus associated with yellowing dwarf disease in China. Arch Virol 167, 983–987 (2022). https://doi.org/10.1007/s00705-022-05360-7
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DOI: https://doi.org/10.1007/s00705-022-05360-7