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Genomic characterization of grapevine viruses N and O: novel vitiviruses from South Africa

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Abstract

A survey was performed on a Vitis cultivar collection in Stellenbosch, South Africa. Metaviromes were generated for each cultivar, using an RNAtag-seq workflow. Analysis of assembled contigs indicated the presence of two putatively novel members of the genus Vitivirus, provisionally named "grapevine virus N" (GVN) and "grapevine virus O" (GVO). Comparisons of amino acid sequences showed that GVN and GVO are most closely related to grapevine virus G and grapevine virus E, respectively. The incidence of these novel viruses within the sampling site was low, with GVO and GVN associated with only five and two cultivars, respectively, of the 229 sampled.

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Funding

David Read is grateful for the financial support provided by the National Research Foundation of South Africa under grant UID 104901.

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Authors and Affiliations

  1. Agricultural Research Council (ARC)—Biotechnology Platform, 100 Old Soutpan Road, Onderstepoort, Pretoria, 0110, South Africa

    David A. Read, Genevieve D. Thompson & Dirk Swanevelder

  2. Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa

    David A. Read & Gerhard Pietersen

  3. Gene Vantage, 34 Monte Carlo Crescent, Kyalami Business Park, Johannesburg, 1684, South Africa

    Genevieve D. Thompson

  4. Patho Solutions, Olifantskop Road, Wellington, 7655, South Africa

    Nathan Le Cordeur

Authors
  1. David A. Read
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  2. Genevieve D. Thompson
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  3. Nathan Le Cordeur
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  4. Dirk Swanevelder
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  5. Gerhard Pietersen
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Correspondence to David A. Read.

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All authors declare that they have no conflict of interest.

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The data that support the findings of this study are openly available in NCBI public databases.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Sead Sabanadzovic.

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Read, D.A., Thompson, G.D., Cordeur, N.L. et al. Genomic characterization of grapevine viruses N and O: novel vitiviruses from South Africa. Arch Virol 167, 611–614 (2022). https://doi.org/10.1007/s00705-021-05333-2

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  • DOI: https://doi.org/10.1007/s00705-021-05333-2

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