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Determination of the complete genomic sequence of grapevine virus H, a novel vitivirus infecting grapevine

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Abstract

The present work reports the discovery and the complete genome sequencing of a novel member of the genus Vitivirus in the family Betaflexiviridae (subfamily Trivirinae) from a symptomless grapevine of unknown variety from Portugal. Total RNAs extracted from phloem scrapings were sequenced using Illumina technology. Bioinformatic analysis of the RNA-seq data revealed a mixed infection involving three viruses and two viroids in addition to a novel vitivirus. Completion and analysis of the genome sequence (7446 nt excluding the polyA tail) showed a typical vitivirus genomic organization. Phylogenetic analysis of the various ORFs clearly showed the new virus to belong in the genus Vitivirus, but sequence divergence firmly establishes it as a member of a new species, for which the name “Grapevine virus H” is proposed.

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Acknowledgements

The authors thank the Platform Genotoul (INRA, Toulouse, France) for the Illumina sequencing.

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Authors and Affiliations

  1. UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, CS 20032, 33882, Villenave d’Ornon Cedex, France

    Thierry Candresse, Sébastien Theil, Chantal Faure & Armelle Marais

Authors
  1. Thierry Candresse
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  2. Sébastien Theil
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  3. Chantal Faure
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  4. Armelle Marais
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Corresponding author

Correspondence to Thierry Candresse.

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All authors (TC, ST, CF and AM) declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: Sead Sabanadzovic.

The nucleotide sequence reported in this work has been deposited in the GenBank database under accession number MF521889.

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Candresse, T., Theil, S., Faure, C. et al. Determination of the complete genomic sequence of grapevine virus H, a novel vitivirus infecting grapevine. Arch Virol 163, 277–280 (2018). https://doi.org/10.1007/s00705-017-3587-7

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  • DOI: https://doi.org/10.1007/s00705-017-3587-7

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