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A new picorna-like virus identified in populations of the potato psyllid Bactericera cockerelli

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Abstract

The potato/tomato psyllid Bactericera cockerelli (Hemiptera: Triozidae) is a pest of Solanaceae plants and a vector of the pathogenic bacterium ‘Candidatus Liberibacter solanacearum’, which is associated with zebra chip disease in potato. This disease is controlled through insecticide treatments, and more environmentally friendly management options are desirable. The objective of this study was to identify viruses present in potato psyllid populations that might be used as biocontrol agents for this insect pest. A new picorna-like virus, tentatively named “Bactericera cockerelli picorna-like virus” (BcPLV), was discovered in B. cockerelli populations maintained in greenhouses, through the use of high-throughput sequencing data and subsequent confirmation by RT-PCR and Sanger sequencing. BcPLV has a positive-sense 9,939-nt RNA genome encoding a single 2,947-aa polyprotein and is related to the Diaphorina citri picorna-like virus (DcPLV) found in Asian citrus psyllid Diaphorina citri populations. Based on their genome organization and the phylogeny of their RNA-dependent RNA polymerase domains, BcPLV and DcPLV together are proposed to comprise a new genus, provisionally named "Psylloidivirus", within the family Iflaviridae.

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Acknowledgements

We thank Erik Wenninger (Kimberly Research and Extension Center, University of Idaho) for providing local psyllids collected in potato fields.

Funding

This research was funded in part through grants from the USDA-ARS, the Idaho State Department of Agriculture Specialty Crop Block Grant Program, USDA-NIFA Hatch Project IDA01560, the ARS/State Potato Partnership Program, and the Idaho Agricultural Experiment Station.

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Author notes

  1. Joseph E. Munyaneza

    Present address: USDA-ARS, Office of National Programs, Crop Production and Protection, 5601 Sunnyside Avenue, Beltsville, MD, 20705-5139, USA

Authors and Affiliations

  1. Department of Entomology, Plant Pathology, and Nematology, University of Idaho, 875 Perimeter Drive, MS 2329, Moscow, ID, 83844-2329, USA

    Jennifer Dahan & Alexander V. Karasev

  2. USDA-ARS, Temperate Tree Fruit and Vegetable Research Unit, Wapato, WA, 98951, USA

    W. Rodney Cooper & Joseph E. Munyaneza

Authors
  1. Jennifer Dahan
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  2. W. Rodney Cooper
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  3. Joseph E. Munyaneza
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  4. Alexander V. Karasev
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Correspondence to Alexander V. Karasev.

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Dahan, J., Cooper, W.R., Munyaneza, J.E. et al. A new picorna-like virus identified in populations of the potato psyllid Bactericera cockerelli. Arch Virol 167, 177–182 (2022). https://doi.org/10.1007/s00705-021-05281-x

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  • DOI: https://doi.org/10.1007/s00705-021-05281-x

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