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A new bipartite begomovirus naturally infecting Pyrenacantha sp. in Mozambique

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Abstract

A begomovirus was isolated from a Pyrenacantha sp. plant with yellow mosaic symptoms collected in a maize production field in Mozambique. The complete DNA-A and DNA-B components have a genomic organization typical of Old World, bipartite begomoviruses. Based on the current ICTV species demarcation criteria for the genus Begomovirus, the virus isolate, named Pyrenacantha yellow mosaic virus (PyYMV), is a member of a new species, for which the name "Begomovirus pyrenacanthae" is proposed. Alignment of their common regions (CR) indicated a 35-nt insertion in the DNA-A CR. The nt sequence identity between the CRs is only 83% but increases to 96% when the 35-nt insertion is removed from the alignment. This is the first report of a begomovirus naturally infecting Pyrenacantha spp.

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Data availability statement

The sequences described in this study were deposited in GenBank under accession numbers MZ390982-MZ390894.

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Acknowledgements

The authors gratefully acknowledge the technical staff at the Fac. de Ciências Agrárias, Unizambeze, for their assistance in sample collection and processing.

Funding

This work was funded by CAPES (financial code 001), CNPq (409599/2016-6) and Fapemig (APQ-03276-18) grants to FMZ. BAIC was supported by a doctoral fellowship from Instituto de Bolsas de Estudos de Moçambique. OFLS was supported by a doctoral fellowship from Ministério da Ciência e Tecnologia, Ensino Superior e Técnico Profissional de Moçambique.

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Authors and Affiliations

  1. Dep. de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil

    Baltazar A. I. Chipiringo, João P. Silva, Renan S. Cascardo, Osvaldo F. L. Sande & F. Murilo Zerbini

  2. Faculdade de Ciências Agrárias, Universidade Zambeze (Unizambeze), Ulónguè, P.O. Box 213, Tête, 2306, Mozambique

    Baltazar A. I. Chipiringo

  3. Divisão de Agricultura, Instituto Superior Politécnico de Manica (DivAG-ISPM), Manica, Mozambique

    Osvaldo F. L. Sande

Authors
  1. Baltazar A. I. Chipiringo
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  2. João P. Silva
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  3. Renan S. Cascardo
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  4. Osvaldo F. L. Sande
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  5. F. Murilo Zerbini
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Correspondence to F. Murilo Zerbini.

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Supplementary Information

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705_2021_5276_MOESM1_ESM.docx

Bayesian phylogenetic tree based on the full-length nucleotide sequences of the DNA-B of PyYMV (marked in red) and related begomoviruses. Labels indicate their abbreviations and GenBank access numbers. Nodes with posterior probability values between 0.5 and 0.79 are indicated by gray circles, and nodes with values equal to or greater than 0.8 are indicated by black circles. The scale bar represents the number of nucleotide substitutions per site. ACMV, African cassava mosaic virus; ACMBFV, African cassava mosaic Burkina Faso virus; AsMMV, asystasia mosaic Madagascar virus; CMMGV, cassava mosaic Madagascar virus; CYMV, cotton yellow mosaic virus; DMV, deinbollia mosaic virus; DesMoV, desmodium mottle virus; EACMV/UG and /TZ, East African cassava mosaic virus Uganda and Tanzania strains, respectively; EACMCMV, East African cassava mosaic Cameroon virus; EACMKV, East African cassava mosaic Kenya virus; EACMZV, East African cassava mosaic Zimbabwe virus; SACMV, South African cassava mosaic virus; SbCBV, soybean chlorotic blotch virus. The tree is rooted with the New World begomovirus bean golden mosaic virus (BGMV).

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Chipiringo, B.A.I., Silva, J.P., Cascardo, R.S. et al. A new bipartite begomovirus naturally infecting Pyrenacantha sp. in Mozambique. Arch Virol 167, 239–243 (2022). https://doi.org/10.1007/s00705-021-05276-8

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