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Complete nucleotide sequence of a novel alphapartitivirus from Rhizoctonia solani AG-4 HG III isolate SM03

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Abstract

In this report, the full genome sequence of a novel mycovirus, designated as "Rhizoctonia solani partitivirus SM03" (RsPV-SM03), was determined in Rhizoctonia solani AG-4 HG III isolate SM03. RsPV-SM03 genome consists of two dsRNAs (dsRNA-1 and dsRNA-2), each of them contains one single open reading frame (ORF). ORF1 of dsRNA-1 encodes a putative RNA-dependent RNA polymerase (RdRp), while ORF2 of dsRNA-2 encodes a putative viral coat protein (CP). Phylogenetic analysis indicated that the RdRp and CP of RsPV-SM03 are closely related to those of other members of the genus Alphapartitivirus, family Partitiviridae, suggesting that RsPV-SM03 represents a novel species in the genus Alphapartitivirus.

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References

  1. Marquez LM, Redman RS, Rodriguez RJ, Roossinck MJ (2007) A virus in a fungus in a plant: three-way symbiosis required for thermal tolerance. Science 315:513–515

    Article  CAS  Google Scholar 

  2. Lyu R, Zhang Y, Tang Q, Li Y, Cheng J, Fu Y, Chen T, Jiang D, Xie J (2018) Two alphapartitiviruses co-infecting a single isolate of the plant pathogenic fungus Rhizoctonia solani. Arch Virol 163:515–520

    Article  CAS  Google Scholar 

  3. Lin YH, Fujita M, Chiba S, Hyodo K, Andika IB, Suzuki N, Kondo H (2019) Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom (Lentinula edodes). Virology 533:125–136

    Article  CAS  Google Scholar 

  4. Xie J, Jiang D (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68

    Article  CAS  Google Scholar 

  5. Zhang T, Li N, Yuan Y, Cao Q, Chen Y, Tan B, Li G, Liu D (2019) Blue-white colony selection of virus-infected isogenic recipients based on a chrysovirus isolated from penicillium italicum. Virol Sin 34:688–700

    Article  CAS  Google Scholar 

  6. Stalpes JA, Andersen TF (1996) A synopsis of the taxonomy of teleomorphs connected with Rhizoctonia S.L. In: Sneh B, Jabaji-Hare S, Neate S, Dijst G (eds) Rhizoctonia species taxonomy, molecular biology, ecology, pathology, and disease control. pp 50–63

  7. Carling DE, Baird RE, Gitaitis RD, Brainard KA, Kuninaga S (2002) Characterization of AG-13, a newly reported anastomosis group of Rhizoctonia solani. Phytopathology 92:893–899

    Article  CAS  Google Scholar 

  8. Sharon M, Sneh B, Kuninaga S, Hyakumachi M (2006) The advancing identification and classification of Rhizoctonia spp. using molecular and biotechnological methods compared with the classical anastomosis grouping. Mycoscience 47:299–316

    Article  CAS  Google Scholar 

  9. DE Carling KS, Brainard KA (2002) Hyphal anastomosis reactions, rDNA-internal transcribed spacer sequences, and virulence levels among subsets of Rhizoctonia solani anastomosis group-2 (AG-2) and AG-BI. Phytopathology 92:43–50

    Article  CAS  Google Scholar 

  10. Bharathan N, Saso H, Gudipati L, Bharathan S, Whited K (2005) Double-stranded RNA: distribution and analysis among isolates of Rhizoctonia solani AG-2 to -13. Plant Pathol 54:196–203

    Article  CAS  Google Scholar 

  11. Zanzinger DH, Bandy BP, Tavantzis SM (1984) High frequency of finding double-stranded RNA in naturally occurring isolates of Rhizoctonia solani. J Gen Virol 65:1601–1605

    Article  CAS  Google Scholar 

  12. Li Y (2015) The establishment of a new anastomosis group AG-V and research of RNA viruses from Rhizoctonia spp., Dissertation, Yunnan Agricultural University

  13. Abdoulaye AH, Foda MF, Kotta-Loizou I (2019) Viruses infecting the plant pathogenic fungus Rhizoctonia solani. Viruses. https://doi.org/10.3390/v11121113

    Article  PubMed  PubMed Central  Google Scholar 

  14. Marzano SL, Nelson BD, Ajayi-Oyetunde O, Bradley CA, Hughes TJ, Hartman GL, Eastburn DM, Domier LL (2016) Identification of diverse mycoviruses through metatranscriptomics characterization of the viromes of five major fungal plant pathogens. J Virol 90:6846–6863

    Article  CAS  Google Scholar 

  15. Zheng L, Zhang M, Chen Q, Zhu M, Zhou E (2014) A novel mycovirus closely related to viruses in the genus Alphapartitivirus confers hypovirulence in the phytopathogenic fungus Rhizoctonia solani. Virology 456–457:220–226

    Article  Google Scholar 

  16. Liu C, Zeng M, Zhang M, Shu C, Zhou E (2018) Complete nucleotide sequence of a partitivirus from Rhizoctonia solani AG-1 IA strain C24. Viruses. https://doi.org/10.3390/v10120703

    Article  PubMed  PubMed Central  Google Scholar 

  17. Zhang M, Zheng L, Liu C, Shu C, Zhou E (2018) Characterization of a novel dsRNA mycovirus isolated from strain A105 of Rhizoctonia solani AG-1 IA. Arch Virol 163:427–430

    Article  Google Scholar 

  18. Strauss EE, Lakshman DK, Tavantzis SM (2000) Molecular characterization of the genome of a partitivirus from the basidiomycete Rhizoctonia solani. J Gen Virol 81:549–555

    CAS  PubMed  Google Scholar 

  19. Chen Y, Gai X,  Chen R, Li C, Zhao G, Xia Z, Zou C, Zhong J (2019) Characterization of three novel betapartitiviruses co-infecting the phytopathogenic fungus Rhizoctonia solani. Virus Res 270:197649

    Article  CAS  Google Scholar 

  20. Morris T, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  21. Darissa O, Willingmann P, Adam G (2010) Optimized approaches for the sequence determination of double-stranded RNA templates. J Virol Methods 169:397–403

    Article  CAS  Google Scholar 

  22. Froussard P (1992) A random-PCR method (rPCR) to construct whole cDNA library from low amounts of RNA. Nucleic Acids Res 20:11

    Article  Google Scholar 

  23. Potgieter AC, Page NA, Liebenberg J, Wright IM, Landt O, van Dijk AA (2009) Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes. J Gen Virol 90:1423–1432

    Article  CAS  Google Scholar 

  24. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  Google Scholar 

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Acknowledgements

This research was supported by Kunming University (YJL16006), Department of Science and Technology of Yunnan Province (2018FH001-032, 2018FH001-052, 2018FD080), and the National Natural Science Foundation of China (31960525).

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Authors and Affiliations

  1. College of Agriculture and Life Sciences, Kunming University, Kunming, 650214, Yunnan, China

    Yanqiong Li, Wurong Chen, Yanfen Niu & Shuhui Yu

  2. Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Yanqiong Li, Ping Xu, Lifang Zhang & Genhua Yang

  3. College of Bioresources and Food Engineering, Qujing Normal University, Qujing, 655011, Yunnan, China

    Lifang Zhang

  4. Yunnan Academy of Tobacco Agricultural Sciences, Kunming, 650021, Yunnan, China

    Yanqiong Li, Ping Xu, Lifang Zhang & Xiaohan Mo

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  1. Yanqiong Li
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  2. Wurong Chen
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  3. Yanfen Niu
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  6. Shuhui Yu
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Correspondence to Genhua Yang or Xiaohan Mo.

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Li, Y., Chen, W., Niu, Y. et al. Complete nucleotide sequence of a novel alphapartitivirus from Rhizoctonia solani AG-4 HG III isolate SM03. Arch Virol 167, 953–957 (2022). https://doi.org/10.1007/s00705-021-05261-1

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  • DOI: https://doi.org/10.1007/s00705-021-05261-1

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