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Characterization of a novel botoulivirus isolated from the phytopathogenic fungus Sclerotinia sclerotiorum

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Abstract

Sclerotinia sclerotiorum ourmiavirus 17 (SsOV17) was isolated from the hypovirulent strain GF3 of Sclerotinia sclerotiorum. The genome of SsOV17 is 2,802 nt in length and contains a single long open reading frame (ORF) flanked by a short structured 5ʹ-untranslated region (5ʹ-UTR) (28 nt) and a long 3ʹ-UTR (788 nt), respectively. The ORF encodes a protein with 663 amino acids and a predicted molecular mass of 75.0 kDa. A BLASTp search indicated that the protein encoded by SsOV17 is closely related to the putative RNA-dependent RNA polymerase (RdRp) of Sclerotinia sclerotiorum ourmiavirus 13 (71% identity). A multiple sequence alignment indicated that eight conserved amino acid motifs were present in the RdRp conserved region of SsOV17. Phylogenetic analysis demonstrated that SsOV17 clustered with members of the genus Botoulivirus.

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Acknowledgements

This research was financially supported by the National Key Research and Development Program of China (2017YFD0201100), the National Natural Science Foundation of China (31772111), and the Earmarked Fund for China Agriculture Research System (CARS-12).

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, The Provincial Key Lab of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Fan Mu, Jichun Jia, Yunxiang Xue, Daohong Jiang, Yanping Fu, Jiasen Cheng, Yang Lin & Jiatao Xie

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  1. Fan Mu
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  2. Jichun Jia
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  3. Yunxiang Xue
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  7. Yang Lin
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  8. Jiatao Xie
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Correspondence to Jiatao Xie.

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Mu, F., Jia, J., Xue, Y. et al. Characterization of a novel botoulivirus isolated from the phytopathogenic fungus Sclerotinia sclerotiorum. Arch Virol 166, 2859–2863 (2021). https://doi.org/10.1007/s00705-021-05168-x

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