Abstract
Sclerotinia sclerotiorum reovirus 1 (SsReV1) was previously reported to infect hypovirulent strain SCH941 of the phytopathogenic fungus Sclerotinia sclerotiorum and to contain 11 double-stranded RNA (dsRNA) segments (S1–S11). Here, we report that SsReV1 is actually composed of 12 dsRNA segments instead of 11. The full-length nucleotide sequence of the twelfth segment (S12) was determined using a combination of RACE and high-throughput sequencing methods. S12 is 1217 nucleotides in length and has highly conserved terminal sequences that resemble those of the other 11 segments of SsReV1. S12 contains a single open reading frame encoding a protein (VP12) of 311 amino acids. Although regular BLAST analysis did not reveal any similarity of VP12 to known sequences, it was found to be homologous to the VP11 of Colorado tick fever virus of the genus Coltivirus when a hidden-Markov-model-based HHpred analysis was performed. A single-protoplast regeneration experiment suggested that S12 and S2 were maintained or lost in parallel. In summary, the SsReV1 genome consists of 12 dsRNA segments.
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References
Hillman BI, Cohen AB (2021) Mycoreoviruses (Reoviridae). In: Bamford DH, Zuckerman M (eds) Encyclopedia of virology, 4th edn. Academic Press, Oxford, pp 607–614. https://doi.org/10.1016/B978-0-12-809633-8.21326-5
Attoui H, Mertens P, Becnel J, Belaganahalli S, Bergoin M, Brussaard C, Chappell J, Ciarlet M, del Vas M, Dermody T et al (2012) Reoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy: Ninth report of the International Committee on Taxonomy of Viruses. Elsevier, San Diego, pp 541–637. https://doi.org/10.1016/B978-0-12-384684-6.00051-3
Aulia A, Andika IB, Kondo H, Hillman BI, Suzuki N (2019) A symptomless hypovirus, CHV4, facilitates stable infection of the chestnut blight fungus by a coinfecting reovirus likely through suppression of antiviral RNA silencing. Virology 533:99–107. https://doi.org/10.1016/j.virol.2019.05.004
Hillman BI, Supyani S, Kondo H, Suzuki N (2004) A reovirus of the fungus Cryphonectria parasitica that is infectious as particles and related to the Coltivirus genus of animal pathogens. J Virol 78:892–898. https://doi.org/10.1128/jvi.78.2.892-898.2004
Kanematsu S, Arakawa M, Oikawa Y, Onoue M, Osaki H, Nakamura H, Ikeda K, Kuga-Uetake Y, Nitta H, Sasaki A (2004) A reovirus causes hypovirulence of Rosellinia necatrix. Phytopathology 94:561–568. https://doi.org/10.1094/PHYTO.2004.94.6.561
Wu S, Cheng J, Fu Y, Chen T, Jiang D, Ghabrial SA, Xie J (2017) Virus-mediated suppression of host non-self recognition facilitates horizontal transmission of heterologous viruses. PLoS Pathog 13:e1006234. https://doi.org/10.1371/journal.ppat.1006234
Bolton MD, Thomma BP, Nelson BD (2006) Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen. Mol Plant Pathol 7:1–16. https://doi.org/10.1111/j.1364-3703.2005.00316.x
Xu L, Liu G, Jiang D, Chen W (2018) Sclerotinia sclerotiorum: an evaluation of virulence theories. Annu Rev Phytopathol 56:311–338. https://doi.org/10.1146/annurev-phyto-080417-050052
Liu L, Cheng J, Fu Y, Liu H, Jiang D, Xie J (2017) New insights into reovirus evolution: implications from a newly characterized mycoreovirus. J Gen Virol 98:1132–1141. https://doi.org/10.1099/jgv.0.000752
Liu L, Wang Q, Cheng J, Fu Y, Jiang D, Xie J (2015) Molecular characterization of a bipartite double-stranded RNA virus and its satellite-like RNA co-infecting the phytopathogenic fungus Sclerotinia sclerotiorum. Front Microbiol 6:406. https://doi.org/10.3389/fmicb.2015.00406
Jia J, Fu Y, Jiang D, Mu F, Cheng J, Lin Y, Li B, Marzano S-YL, Xie J (2021) Interannual dynamics, diversity and evolution of the virome in Sclerotinia sclerotiorum from a single crop field. Virus Evol 7:veab32. https://doi.org/10.1093/ve/veab032
Potgieter AC, Page NA, Liebenberg J, Wright IM, Landt O, van Dijk AA (2009) Improved strategies for sequence-independent amplification and sequencing of viral double-stranded RNA genomes. J Gen Virol 90:1423–1432. https://doi.org/10.1099/vir.0.009381-0
Attoui H, Jaafar FM, Biagini P, Cantaloube J-F, De Micco P, Murphy F, De Lamballerie X (2002) Genus Coltivirus (family Reoviridae): genomic and morphologic characterization of Old World and New World viruses. Arch Virol 147:533–561. https://doi.org/10.1007/s007050200005
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This research was financially supported by the National Natural Science Foundation of China (31772111) and the China Agriculture Research System of MOF and MARA.
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Jia, J., Mu, F., Fu, Y. et al. Characterization of a newly identified RNA segment derived from the genome of Sclerotinia sclerotiorum reovirus 1. Arch Virol 167, 603–606 (2022). https://doi.org/10.1007/s00705-021-05319-0
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DOI: https://doi.org/10.1007/s00705-021-05319-0