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Characterization and genome sequence of the genetically unique Escherichia bacteriophage vB_EcoM_IME392

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Abstract

In this study, a novel Escherichia coli-specific bacteriophage, vB_EcoM_IME392, was isolated from chicken farm sewage in Qingdao, China. The genome of IME392 was found by next-generation sequencing to be 116,460 base pairs in length with a G+C content of 45.4% (GenBank accession number MH719082). BLASTn results revealed that only 2% of the genome sequence of IME392 shows sequence similarity to known phage sequences in the GenBank database, which indicates that IME392 is a novel bacteriophage. Transmission electron microscopy showed that IME392 belongs to the family Myoviridae. The host range, the multiplicity of infection, and a one-step growth curve were also determined.

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Acknowledgements

This research was supported by the Key Project of Beijing University of Chemical Technology (XK1803-06), the National Key Research and Development Program of China (2018YFA0903000, 2020YFC2005405, 2020YFA0712100, 2020YFC0840805), Funds for First-Class Discipline Construction (XK1805), the Inner Mongolia Key Research and Development Program (2019ZD006), the National Natural Science Foundation of China (81672001, 81621005), the NSFC-MFST Project (China-Mongolia) (31961143024), and Fundamental Research Funds for Central Universities (BUCTRC201917, BUCTZY2022).

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    Authors and Affiliations

    1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

      Yunjia Hu, Xiaoping An, Lihua Song, Huahao Fan & Yigang Tong

    2. Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China

      Lihua Song, Huahao Fan & Yigang Tong

    3. Bioinformatics Graduate Program, University of British Columbia, Vancouver, BC, Canada

      Shanwei Tong

    4. College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China

      Ping Li

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    Hu, Y., Tong, S., Li, P. et al. Characterization and genome sequence of the genetically unique Escherichia bacteriophage vB_EcoM_IME392. Arch Virol 166, 2505–2520 (2021). https://doi.org/10.1007/s00705-021-05160-5

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