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Activation of the extracellular signal-regulated kinase pathway is required for replication of Newcastle disease virus

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Abstract

Replication of Newcastle disease virus (NDV) is regulated by various host mechanisms, but the role of the extracellular signal-regulated kinase (ERK) pathway in regulating NDV replication is an open question. In this study, the relationship between the ERK pathway and NDV replication was investigated. NDV activated the ERK signaling in chicken embryo fibroblasts at the late stage of infection, correlating to expression of viral proteins. Specific blockage of the ERK pathway activation significantly decreased the transcription and translation levels of viral genes as well as virus replication and the cytopathogenic effect caused by NDV. Our results demonstrate that activation of the ERK pathway is required for NDV replication.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31702243), the Earmarked Fund for China Agriculture Research System (CARS-40), and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. Key Laboratory of Animal Infectious Diseases, School of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China

    Jie Ni, Shunlin Hu, Xiaoquan Wang & Xiaowen Liu

  2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China

    Jie Ni, Shunlin Hu, Xiaoquan Wang, Xiaowen Liu, Zenglei Hu & Xiufan Liu

  3. Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-Food Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, 225009, China

    Shunlin Hu, Xiaoquan Wang & Xiaowen Liu

  4. Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, No. 12, East Wenhui Rd, Yangzhou, 225009, Jiangsu, China

    Shunlin Hu, Xiaoquan Wang, Xiaowen Liu, Zenglei Hu & Xiufan Liu

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  1. Jie Ni
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  2. Shunlin Hu
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  3. Xiaoquan Wang
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Correspondence to Zenglei Hu or Xiufan Liu.

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Experiments involving virulent NDVs were performed in biosafety level 3 animal facilities.

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Ni, J., Hu, S., Wang, X. et al. Activation of the extracellular signal-regulated kinase pathway is required for replication of Newcastle disease virus. Arch Virol 166, 921–927 (2021). https://doi.org/10.1007/s00705-020-04938-3

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